RELATIONSHIPS BETWEEN MYCOPLASMA-PNEUMONIAE AND HUMAN RESPIRATORY EPITHELIUM
RELATIONSHIPS BETWEEN MYCOPLASMA-PNEUMONIAE AND HUMAN RESPIRATORY EPITHELIUM
复制标题
DOI：
10.1128/iai.3.5.694-701.1971
复制
发表时间：
1971-01-01
影响因子：
3.1
通讯作者：
CLYDE, WA
中科院分区：
文献类型：
--
作者：
COLLIER, AM;CLYDE, WA
研究了肺炎支原体与其天然宿主细胞人呼吸道上皮细胞之间的相互作用。有组织的，纤毛细胞提供的胎儿气管在器官培养，使检查的宿主-寄生虫的关系，通过光，免疫荧光和电子显微镜。纤毛运动紊乱和丧失反映了细胞功能受损;这与一系列细胞病理学变化相关，表明细胞损伤进行性。发现微生物集中在纤毛上皮的腔表面和粘膜下腺体的细胞内衬上。支原体的一个分化部分，由一个含有电子致密核的单位膜的延伸部分组成，周围有一个透明的空间，作为附着到宿主细胞的手段。结果表明，M.肺炎依赖于由宿主细胞膜损伤引起的功能和结构变化的细胞外感染。
The interaction was studied betweenMycoplasma pneumoniaeand its natural host cell, the human respiratory epithelium. Organized, ciliated cells provided by fetal trachea in organ culture enabled examination of the host-parasite relationship by light, immunofluorescence, and electron microscopy. Impairment of cellular function was reflected by disorganization and loss of ciliary motion; this was associated with a sequence of cytopathological changes denoting progressive cell injury. The organisms were found concentrated on the luminal surface of ciliated epithelium and cells lining the submucosal glands. A differentiated portion of theMycoplasma, consisting of an extension of the unit membrane containing an electron-dense core surrounded by a lucent space, served as the means of attachment to host cells. The findings suggest that the pathogenicity ofM. pneumoniaedepends upon intimate extracellular infection with production of functional and structural changes initiated by host cell membrane injury.