Permeability of injured and intact peripheral nerves and dorsal root ganglia
Permeability of injured and intact peripheral nerves and dorsal root ganglia
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DOI：
10.1097/00000542-200607000-00024
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发表时间：
2006-07-01
期刊：
影响因子：
8.8
通讯作者：
Hogan, Quinn H.
中科院分区：
文献类型：
--
作者：
Abram, Stephen E.;Yi, Johnny;Hogan, Quinn H.
背景：神经损伤引起动物异常性痛觉和痛觉过敏的行为改变与背根神经节（DRG）细胞的电生理改变有关。将改变钙、钠或钾通道活性的药物引入DRG或周围神经可能对神经损伤后的治疗有益。出于这个原因，作者试图确定通常不通过血神经屏障的药物在静脉注射或局部注射后是否会进入DRG，并确定神经损伤是否会改变药物进入DRG或周围神经的途径。方法：采用静脉注射、鞘内注射、drg周围注射、硬膜外注射荧光素钠给药。取DRG、坐骨神经和脊髓组织冷冻，定量分析组织荧光。结果：在完整和神经损伤的动物中，荧光素在静脉注射、drg周围和硬膜外注射后均在drg中积累。鞘内注射后神经节近端有堆积。经静脉注射或神经周围注射后，在完整动物的坐骨神经中发现少量荧光素，但在神经损伤动物的某些神经束中，经静脉注射和神经周围注射均发现大量荧光素。除鞘内给药外，脊髓中几乎未发现荧光素。结论：在完整和神经损伤的动物中，荧光素在静脉注射、硬膜外注射或椎旁注射后在DRG中自由积聚。坐骨神经对荧光素是相对不渗透的，但在神经损伤后，通过全身或局部注射可增强其进入。
Background: Nerve injury that produces behavioral changes of allodynia and hyperalgesia in animals is associated with electrophysiologic changes in dorsal root ganglion (DRG) cells. The introduction of drugs into the DRG or the peripheral nerve that alter calcium, sodium, or potassium channel activity may be of therapeutic benefit after nerve injury. For this reason, the authors sought to determine whether drugs that do not ordinarily cross the blood-nerve barrier will enter the DRG after intravenous or regional injection and to determine whether nerve injury alters drug access to DRGs or peripheral nerves.Methods: Both intact and spinal nerve-ligated rats were injected with sodium fluorescein by intravenous, intrathecal, peri-DRG, perisciatic, and epidural routes. DRG, sciatic nerve, and spinal cord tissues were harvested and frozen, and histologic sections were analyzed quantitatively for tissue fluorescence.Results: In both intact and nerve-injured animals, fluorescein accumulated in DRGs after intravenous, peri-DRG, and epidural injection. There was accumulation in the proximal portion of the ganglion after intrathecal injection. Minimal amounts of fluorescein were found in the sciatic nerve in intact animals after intravenous or perineural injection, but substantial amounts were found in some nerve fascicles in nerve-injured animals after both intravenous and perineural injection. There was almost no fluorescein found in the spinal cord except after intrathecal administration.Conclusions: In both intact and nerve-injured animals, fluorescein accumulates freely in the DRG after intravenous, epidural, or paravertebral injection. The sciatic nerve is relatively impermeable to fluorescein, but access by either systemic or regional injection is enhanced after nerve injury.