Genetic and antigenic characterization of H5 and H7 influenza viruses isolated from migratory water birds in Hokkaido, Japan and Mongolia from 2010 to 2014
Genetic and antigenic characterization of H5 and H7 influenza viruses isolated from migratory water birds in Hokkaido, Japan and Mongolia from 2010 to 2014
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DOI：
10.1007/s11262-015-1214-9
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发表时间：
2015-08-01
期刊：
影响因子：
1.6
通讯作者：
Kida, Hiroshi
中科院分区：
文献类型：
--
作者：
Hiono, Takahiro;Ohkawara, Ayako;Kida, Hiroshi
迁徙水鸟是甲型流感病毒的天然宿主。H5和H7流感病毒在世界各地被分离出来，也在亚洲的家禽中传播。2010年，在日本北海道从俄罗斯西伯利亚向南迁徙的水鸟粪便中分离到2株H5 N1型高致病性禽流感病毒（HPAIV）。H7N9病毒在中国从人类中零星分离，并在家禽中传播。为了监测这些病毒是否在野鸟种群中传播，我们在2010年至2014年秋季对迁徙水鸟进行了禽流感病毒学监测。在日本和蒙古共采集迁徙水鸟粪便样本8103份，分离到流感病毒350株，其中H5型13株，H7型19株。系统发育分析显示，所有分离株在遗传上与野生水鸟中流行的病毒密切相关。抗原性分析结果表明，野生水禽中病毒的抗原性具有高度稳定性，但与亚洲分离的高致病性禽流感病毒不同。目前的结果表明，HPAIV和中国H7N9病毒并不是主要在候鸟中传播;然而，建议继续监测家禽和野生鸟类中的H5和H7流感病毒，以控制禽流感。
Migratory water birds are the natural reservoir of influenza A viruses. H5 and H7 influenza viruses are isolated over the world and also circulate among poultry in Asia. In 2010, two H5N1 highly pathogenic avian influenza viruses (HPAIVs) were isolated from fecal samples of water birds on the flyway of migration from Siberia, Russia to the south in Hokkaido, Japan. H7N9 viruses are sporadically isolated from humans and circulate in poultry in China. To monitor whether these viruses have spread in the wild bird population, we conducted virological surveillance of avian influenza in migratory water birds in autumn from 2010 to 2014. A total of 8103 fecal samples from migratory water birds were collected in Japan and Mongolia, and 350 influenza viruses including 13 H5 and 19 H7 influenza viruses were isolated. A phylogenetic analysis revealed that all isolates are genetically closely related to viruses circulating among wild water birds. The results of the antigenic analysis indicated that the antigenicity of viruses in wild water birds is highly stable despite their nucleotide sequence diversity but is distinct from that of HPAIVs recently isolated in Asia. The present results suggest that HPAIVs and Chinese H7N9 viruses were not predominantly circulating in migratory water birds; however, continued monitoring of H5 and H7 influenza viruses both in domestic and wild birds is recommended for the control of avian influenza.