Label-Free, Real-Time Multiplexed DNA Detection Using Fluorescent Conjugated Polymers
Label-Free, Real-Time Multiplexed DNA Detection Using Fluorescent Conjugated Polymers
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DOI：
10.1021/ja809175q
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发表时间：
2009-03-18
影响因子：
15
通讯作者：
He, Lin
中科院分区：
文献类型：
--
作者：
Zheng, Weiming;He, Lin
一种无标记，多重DNA检测使用荧光共轭聚合物作为检测探针，以说明杂交金属条纹纳米棒的证明。不同的DNA捕获探针由Au和Ag条带图案的不同反射率编码。成功的DNA杂交诱导共轭聚噻吩衍生物从形成单链DNA-聚合物复合物到形成双链DNA-聚合物复合物的光学可检测的构象变化。结果显示阿托摩尔检测灵敏度和单突变特异性与单元素测定相当，但具有更高的通量。
A label-free, multiplexed DNA assay using fluorescent conjugated polymers as a detection probe to illustrate hybridization on metallic striped nanorods is demonstrated. Different DNA capture probes were encoded by the different reflectivities of Au and Ag stripe patterns. Successful DNA hybridization induced an optically detectable conformational change in conjugated polythiophene derivatives from forming single-stranded DNA-polymer complexes to forming double-stranded DNA-polymer ones. The results show attomole detection sensitivity and single-mutation specificity comparable to those of single-element assays but with much improved throughput.