Transcriptome analysis of the male polymorphisms of fig wasp species Philotrypesis tridentata
Transcriptome analysis of the male polymorphisms of fig wasp species Philotrypesis tridentata
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无花果蜂种 Philotrypesis tridentata 雄性多态性的转录组分析
DOI：
10.1016/j.ijbiomac.2020.07.294
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发表时间：
2020-12-01
影响因子：
8.2
通讯作者：
Huang，Da-Wei
中科院分区：
文献类型：
--
作者：
Xin，Zhao-Zhe;Hou，Hong-Xia;Huang，Da-Wei
种内雄性多态性在形态、行为和生活史上的极端差异为探索不同环境的适应机制提供了良好的机会。在这项研究中，我们研究了无翅和有翅无花果黄蜂的转录组学差异，以探讨维持多态性的分子基础。有翼的成年雄性飞到无花果树外交配，而无翼的只在它们发育的无花果树内交配。我们鉴定出2391个差异表达基因（DEGs），其中1396个在有翼变形中高表达，995个在无翼变形中高表达。我们对deg和差异选择性剪接基因进行了基因本体（Gene Ontology）和京都基因百科全书（Kyoto Encyclopedia of Genes and Genomes）富集分析，并分析了每种形态中差异表达最高的前10个deg。结果表明，与无花果果外复杂环境的生物合成过程、脂质代谢、能量产生、飞行和防御相关的基因在有翼形态中表达上调。无翅异构体中参与物质和能量代谢及化学物质接受的基因表达上调，这可能与它们在无花果体内生活有关。高表达基因的差异证明了p的适应性。三叉蝽在不同生存环境下的多态性。
Intraspecific male polymorphism exhibiting extreme differences in morphology, behavior and life history presents good opportunities to explore adaptation mechanisms to different environments. In this study, we examined the transcriptomic differences between wingless and winged morphs of a fig wasp speciesPhilotrypesis tridentatato investigate molecular basis to maintain polymorphisms. The winged male adults fly outside fig syconia to mate, while the wingless only stay and mate inside fig syconia where they have developed. We identified 2,391 differentially expressed genes (DEGs) with 1,396 highly expressed in winged morphs and 995 in wingless morphs. We performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses on the DEGs and differential alternative splicing genes and analyzed the top ten DEGs with the highest differential expression in each morph. The results showed that genes related to biosynthesis processes, lipid metabolism, energy production, flight and defense of the complex environments outside fig syconia were up-regulated in winged morphs. Genes involved in substance and energy metabolism and chemical reception were up-regulated in wingless morphs which might relate to their living inside fig syconia. The differences in highly expressed genes between two morphs prove adaptation ofP. tridentatamale polymorphism to different living environments.