Localisation of vascular endothelial growth factor and its receptors to cells of vascular and avascular epiretinal membranes
Localisation of vascular endothelial growth factor and its receptors to cells of vascular and avascular epiretinal membranes
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DOI：
10.1136/bjo.81.10.919
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发表时间：
1997-10-01
影响因子：
4.1
通讯作者：
Campochiaro, PA
中科院分区：
文献类型：
--
作者：
Chen, YS;Hackett, SF;Campochiaro, PA
目的/背景-视网膜前膜（ERM）的产生有多种原因，或在某些情况下，原因不明。一旦建立，ERMs倾向于进展，变得更广泛，并沿着视网膜的内表面施加沿着增加的牵引力。其进展的一个可能原因是ERMs内的细胞产生生长因子，其可提供自分泌或旁分泌刺激。血小板衍生生长因子（PDGF）及其受体已定位于ERMs细胞，并可能发挥这种作用。在这项研究中，比较数据，寻求其他几个生长因子，已牵连在ERM formation. Methods免疫组化染色的ERMs进行了PDGF-A，PDGF-B，碱性成纤维细胞生长因子（bFGF），三种亚型的转化生长因子β（TGF-β），血管内皮生长因子（VEGF）及其受体，Flt-1和flk-1/KDR。Flt-1和flk-1/KDR的表达进行了研究，在培养的视网膜色素上皮（RPE）细胞和视网膜胶质细胞从死后的眼睛免疫组化和逆转录聚合酶链反应（RT-PCR）。结果染色是最强烈的，最常见的VEGF和PDGF-A，无论是在血管和无血管的ERMs。11例糖尿病视网膜病变中9例（81.8%）和24例增生性玻璃体视网膜病变中14例（58.3%）的大部分细胞为VEGF染色。还在ERM中的细胞和培养的RPE细胞上鉴定了VEGF、flt-1和flk-1/KDR的受体。通过RT-PCR，mRNA的flt-1被确定在RPE细胞和视网膜神经胶质细胞，和mRNA的flk-1/KDR被确定在RPE cells. Conclusions-这些数据表明，VEGF及其受体被本地化到血管和无血管的ERMs，并建议VEGF，像PDGF-A，可能是一个自分泌和旁分泌刺激，可能有助于血管和无血管的ERMs的进展。
Aims/background-Epiretinal membranes (ERMs) arise from a variety of causes or, in some cases, for unknown reasons. Once established, ERMs tend to progress, becoming more extensive and exerting increasing traction along the inner surface of the retina. One possible cause for their progression is the production of growth factors by cells within ERMs that may provide autocrine or paracrine stimulation. Platelet derived growth factor (PDGF) and its receptors have been localised to cells of ERMs and may play such a role. In this study, comparative data were sought for several other growth factors that have been implicated in ERM formation.Methods-Immunohistochemical staining of ERMs was done for PDGF-A, PDGF-B, basic fibroblast growth factor (bFGF), three isoforms of transforming growth factor beta (TGF-beta), and vascular endothelial growth factor (VEGF) and its receptors, flt-1 and flk-1/KDR. Expression of flt-1 and flk-1/KDR was examined in cultured retinal pigmented epithelial (RPE) cells and retinal glia from postmortem eyes by immunohistochemistry and by reverse transcription coupled to polymerase chain reaction (RT-PCR).Results-Staining was most intense and most frequently observed for VEGF and PDGF-A, both in vascular and avascular ERMs. The majority of cells stained for VEGF in nine of 11 (81.8%) diabetic ERMs and in 14 of 24 (58.3%) proliferative vitreoretinopathy ERMs. The receptors for VEGF, flt-1, and flk-1/KDR were also identified on cells in ERMs and on cultured RPE cells. By RT-PCR, mRNA for flt-1 was identified in RPE cells and retinal glia, and mRNA for flk-1/KDR was identified in RPE cells.Conclusions-These data show that VEGF and its receptors are localised to both vascular and avascular ERMs and suggest that VEGF, like PDGF-A, may be an autocrine and paracrine stimulator that may contribute to progression of vascular and avascular ERMs.