Genetic variation of Ginkgo biloba L. (Ginkgoaceae) based on cpDNA PCR-RFLPs:: inference of glacial refugia
Genetic variation of Ginkgo biloba L. (Ginkgoaceae) based on cpDNA PCR-RFLPs:: inference of glacial refugia
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DOI：
10.1038/sj.hdy.6800616
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发表时间：
2005-04-01
期刊：
影响因子：
3.8
通讯作者：
Qiu, YX
中科院分区：
文献类型：
--
作者：
Shen, L;Chen, XY;Qiu, YX
银杏（Ginkgo biloba）是银杏属唯一的生存者，是著名的活化石。为了进一步推断冰川区是否存在巨藻，我们利用叶绿体DNA （cpDNA）检测了8个潜在的巨藻避难种群和人工林的遗传结构，并在trnK1-trnK2片段中发现了8个大小变异。数据包括来自8个地区的158棵树的单倍型。大多数cpDNA单倍型局限于地理范围的一小部分。结果表明，中国西南地区是双叶大蠊的避难所。该地区是目前具有全球重要性的生物多样性热点地区，可能在更新世期间受到极端气候波动的保护。西天目山的银杏，以前被许多研究人员认为是野生的，可能是佛教僧侣引进的。
Ginkgo biloba, a famous living fossil, is the sole survivor of the genus Ginkgo. To make inferences about the glacial refugia that harbored G. biloba, we examined the genetic structure of eight potential refugial populations and plantations using chloroplast DNA (cpDNA) with eight size variants in the trnK1-trnK2 fragment. The data consist of haplotypes from 158 trees collected from eight localities. The majority of the cpDNA haplotypes are restricted to minor portions of the geographical range. Our results suggest that refugia of G. biloba were located in southwestern China. This area is a current biodiversity hotspot of global importance, and may have been protected from the extremes of climatic fluctuations during the Pleistocene. The Ginkgos on West Tianmu Mountain, which were previously considered to be wild by many researchers, may, instead, have been introduced by Buddhist monks.