Mesenchymal stem cell transplantation accelerates hearing recovery through the repair of injured cochlear fibrocytes
Mesenchymal stem cell transplantation accelerates hearing recovery through the repair of injured cochlear fibrocytes
复制标题
DOI：
10.2353/ajpath.2007.060948
复制
发表时间：
2007-07-01
影响因子：
6
通讯作者：
Matsunaga, Tatsuo
中科院分区：
文献类型：
--
作者：
Kamiya, Kazusaku;Fujinami, Yoshiaki;Matsunaga, Tatsuo
耳蜗肌纤维细胞在正常听力以及由内耳内环境平衡失调引起的几种感音神经性听力损失中起着重要作用。最近，我们建立了一种新的大鼠急性感音神经性听力损失模型，该模型可归因于线粒体毒素引起的纤维细胞功能障碍。在这个模型中，我们展示了在严重的局灶性细胞凋亡后耳蜗肌纤维细胞的活跃再生，而Corti器官没有任何变化。为了挽救残留的听力损失，我们将间充质干细胞移植到外侧半规管，然后在损伤的侧壁区域检测到大量的干细胞。移植间充质的大鼠。侧壁干细胞的听力恢复率明显高于对照组。间充质细胞。干细胞的侧壁也显示连接蛋白26和连接蛋白30的免疫染色使人联想到。相邻细胞之间的缝隙连接。这些结果表明，在该模型中，耳蜗纤维细胞的重组可导致急性感音神经性聋后听力的恢复，提示间充质干细胞内耳内移植可能是治疗因耳蜗纤维细胞变性所致的感音神经性聋的一种有前途的治疗方法。
Cochlear fibrocytes play important roles in normal hearing as well as in several types of sensorineural hearing loss attributable to inner ear homeostasis disorders. Recently, we developed a novel rat model of acute sensorineural hearing loss attributable to fibrocyte dysfunction induced by a mitochondrial toxin. in this model, we demonstrate active regeneration of the cochlear fibrocytes after severe focal apoptosis without any changes in the organ of Corti. To rescue the residual hearing loss, we transplanted mesenchymal stem cells into the lateral semicircular canal, a number of these stem cells were then detected in the injured area in the lateral wall. Rats with transplanted mesenchymal. stem cells in the lateral wall demonstrated a significantly higher hearing recovery ratio than controls. The mesenchymal. stem cells in the lateral wall also showed connexin 26 and connexin 30 immunostaining reminiscent of. gap junctions between neighboring cells. These results indicate that reorganization of the cochlear fibrocytes leads to hearing recovery after acute sensorineural hearing loss in this model and suggest that mesenchymal stem cell trans plantation into the inner ear may be a promising therapy for patients with sensorineural hearing loss attributable to degeneration of cochlear fibrocytes.