NERVE GROWTH-FACTOR AND PREGANGLIONIC CHOLINERGIC NERVES - THEIR RELATIVE IMPORTANCE TO DEVELOPMENT OF TERMINAL ADRENERGIC NEURON
NERVE GROWTH-FACTOR AND PREGANGLIONIC CHOLINERGIC NERVES - THEIR RELATIVE IMPORTANCE TO DEVELOPMENT OF TERMINAL ADRENERGIC NEURON
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DOI：
10.1016/0006-8993(72)90321-6
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发表时间：
1972-01-01
期刊：
影响因子：
2.9
通讯作者：
KETTLER, R
中科院分区：
文献类型：
--
作者：
THOENEN, H;SANER, A;KETTLER, R
为阐明神经生长因子（NGF）和节前胆碱能神经活性在终末肾上腺素能神经元发育中的相对重要性，我们观察了大鼠颈上级神经节在切断节前胆碱能神经干后生长分化的变化及其对NGF反应的变化。老年Wistar大鼠，雌雄各半。两周后测定胆碱乙酰转移酶活性作为分散完全性的量度，蛋白质含量作为一般生长的量度，酪氨酸羟化酶（TH）和多巴胺β-羟化酶（DBH）的比活性（形成的产物/h/mg蛋白）阿萨终末肾上腺素能神经元分化的量度。去中心化侧胆碱乙酰基转移酶（Choline Acetyltransferase，CAT）活性降至完整侧的3%以下，蛋白质含量降至68 ± 3%，TH比活性降至80 ± 6%，DBH比活性降至79 ± 8%，去中心化不影响NGF对整体生长的影响，也不影响TH和DBH的选择诱导作用。结论：终末肾上腺素能神经元生长分化的主要因素是神经生长因子，它不依赖于节前胆碱能纤维的完整性。拿铁咖啡的活性似乎只是肾上腺素能神经元发育的一个附加因素。
To delineate the relative importance of nerve growth factor (NGF) and the activity of preganglionic cholinergic nerves for the development of the terminal adrenergic neuron we have studied the changes in growth and differentiation of the rat superior cervical ganglion and the changes in its response to NGF after dissection of the preganglionic cholinergic trunk.Decentralization of the left superior cervical ganglion was performed in 3-day-old Wistar rats of either sex. Two weeks later the choline acetyltransferase activity was determined as a measure of completeness of decentralization, the protein content as a measure of general growth and the specific activity (product formed/h/mg protein) of tyrosine hydroxylase (TH) and dopamine β-hydroxylase (DBH) asa measure of differentiation of the terminal adrenergic neuron. Choline acetyltransferase of the decentralized side was reduced to < 3% of that of the intact side, the protein content to 68 ± 3%, the specific activity of TH to 80 ± 6%, and that of DBH to 79 ± 8%.Decentralization did not impair the effect of NGF on general growth or the selective induction of TH and DBH. It is concluded that the main factor for growth and differentiation of the terminal adrenergic neuron is NGF, which does not depend on the intactness of the preganglionic cholinergic fibers. The activity of the latte seems to represent an additive factor only to the development of the adrenergic neuron.