Application of External Force Regulates the Migration and Differentiation of Adipose-Derived Stem/Progenitor Cells by Altering Tissue Stiffness
Application of External Force Regulates the Migration and Differentiation of Adipose-Derived Stem/Progenitor Cells by Altering Tissue Stiffness
复制标题
外力的应用通过改变组织硬度来调节脂肪干细胞/祖细胞的迁移和分化
DOI：
10.1089/ten.tea.2019.0046
复制
发表时间：
2019-11-20
影响因子：
4.1
通讯作者：
Dong, Ziqing
中科院分区：
文献类型：
--
作者：
Li, Ye;Wu, Mengfan;Dong, Ziqing
大的软组织缺损是具有挑战性的重建手术。使用外部容积扩张（EVE）器械扩张软组织是改善此类重建的非侵入性方法;然而，其潜在机制尚不清楚。在这项研究中，我们在Sprague-Dawley大鼠中创建脂肪瓣，使用EVE装置施加3或6 kPa的外力，并研究脂肪来源的干/祖细胞（ASCs）的迁移和分化。此外，我们进行了有限元分析，以探讨脂肪组织的刚度。3 kPa的外力促进ASCs的迁移和成脂分化。相比之下，6 kPa的外力对ASCs迁移的影响较大，但对ASCs成脂分化的影响较小。外力影响脂肪组织硬度。总之，由EVE装置产生的外力增加了脂肪组织的硬度，这影响了ASC的迁移和分化。可以根据特定时间点所需的组织硬度来改变外力的大小，以促进脂肪组织的长期再生。
Large soft-tissue defects are challenging to reconstruct surgically. Expansion of soft tissue using an external volume expansion (EVE) device is a noninvasive method to improve such reconstruction; however, the underlying mechanism is unclear. In this study, we created fat flaps in Sprague-Dawley rats, applied an external force of 3 or 6 kPa using an EVE device, and investigated the migration and differentiation of adipose-derived stem/progenitor cells (ASCs). In addition, we performed finite element analysis to explore the stiffness of adipose tissue. An external force of 3 kPa promoted the migration and adipogenic differentiation of ASCs. By comparison, an external force of 6 kPa had a larger effect on migration of ASCs, but a smaller effect on adipogenic differentiation of ASCs. External force affected adipose tissue stiffness. In conclusion, external force generated by an EVE device increases the stiffness of adipose tissue, which influences the migration and differentiation of ASCs. The size of the external force can be altered according to the tissue stiffness required at particular time points to promote long-term adipose tissue regeneration.