Nitric oxide within the ventral tegmental area is involved in mediating morphine reward
Nitric oxide within the ventral tegmental area is involved in mediating morphine reward
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DOI：
10.1016/s0014-2999(02)02696-1
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发表时间：
2003-01-01
影响因子：
5
通讯作者：
Haerri-Rohani, A
中科院分区：
文献类型：
--
作者：
Gholami, A;Zarrindast, MR;Haerri-Rohani, A
本研究观察了腹侧被盖区注射一氧化氮（NO）前体L-精氨酸和一氧化氮合酶（NOS）抑制剂N-硝基-L-精氨酸甲酯（L-NAME）对吗啡诱导的雄性Wistar大鼠条件性位置偏爱的影响。我们的数据表明，皮下（s.c.）注射硫酸吗啡（0.5-10 mg/kg）以剂量依赖性方式显著增加了在药物配对隔室中花费的时间。内腹侧被盖区管理的低剂量的L-精氨酸（0.05微克/大鼠）与无效剂量的吗啡（0.5毫克/公斤）引起显着的条件性位置偏爱，然而，较高剂量的L-精氨酸（0.1微克/大鼠）减少吗啡的反应。腹侧被盖区给予L-NAME（0.03和0.1 μ g/大鼠）可降低吗啡（7.5 mg/kg）诱导的位置偏爱的获得。L-NAME（0.03 μ g/大鼠）可降低对不同剂量L-精氨酸的反应。L-精氨酸和L-NAME本身没有引起任何影响的位置条件反射，但是，腹侧被盖区的L-精氨酸（0.01-0.1 μ g/只）和更高剂量的L-NAME（0.1 μ g/只）显着降低吗啡（7.5毫克/公斤）诱导的位置偏爱的表达。L-NAME（0.03 μ g/大鼠）可抑制已建立的吗啡诱导的位置偏爱在试验日由L-精氨酸引起的衰减。提示NO可能参与吗啡诱导的位置偏爱的获得和表达。(C)2002 Elsevier Science B. V.保留所有权利。
In the present study, the effects of intra-ventral tegmental area injection of L-arginine, a nitric oxide (NO) precursor, and N-G-nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase (NOS) inhibitor, on morphine-induced conditioned place preference in male Wistar rats were investigated. Our data showed that subcutaneous (s.c.) injection, of morphine sulphate (0.5-10 mg/kg) significantly increased the time spent in the drug-paired compartment in a dose-dependent manner. Intra-ventral tegmental area administration of a low dose of L-arginine (0.05 mug/rat) with an ineffective dose of morphine (0.5 mg/kg) elicited significant conditioned place preference; however, a higher dose of L-arginine (0.1 mug/rat) reduced the morphine response. Intra-ventral tegmental area administration of L-NAME (0.03 and 0.1 mug/rat) decreased the acquisition of morphine (7.5 mg/kg)-induced place preference. The response to different doses of L-arginine was decreased by L-NAME (0.03 mug/rat). L-Arginine and L-NAME by themselves did not elicit any effect on place conditioning; however, intra-ventral tegmental area administration of L-arginine (0.01-0.1 mug/rat) and a higher dose of L-NAME (0.1 mug/rat) significantly decreased the expression of morphine (7.5 mg/kg)-induced place preference. The attenuation of already established morphine-induced place preference on the test day by L-arginine was inhibited by L-NAME (0.03 mug/rat). The results indicate that NO may be involved in the acquisition and expression of morphine-induced place preference. (C) 2002 Elsevier Science B.V. All rights reserved.