PNEUMOCOCCAL SURFACE PROTEIN-A (PSPA) IS SEROLOGICALLY HIGHLY VARIABLE AND IS EXPRESSED BY ALL CLINICALLY IMPORTANT CAPSULAR SEROTYPES OF STREPTOCOCCUS-PNEUMONIAE
PNEUMOCOCCAL SURFACE PROTEIN-A (PSPA) IS SEROLOGICALLY HIGHLY VARIABLE AND IS EXPRESSED BY ALL CLINICALLY IMPORTANT CAPSULAR SEROTYPES OF STREPTOCOCCUS-PNEUMONIAE
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DOI：
10.1128/iai.58.10.3293-3299.1990
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发表时间：
1990-10-01
影响因子：
3.1
通讯作者：
BRILES, DE
中科院分区：
文献类型：
--
作者：
CRAIN, MJ;WALTMAN, WD;BRILES, DE
肺炎球菌表面蛋白A（PspA）以前已被证明可以引发针对肺炎球菌感染的保护性抗体，并且是小鼠中完全肺炎球菌毒力所必需的。该蛋白最初由两种小鼠单克隆抗体Xi64和Xi126定义，它们共同识别14%的肺炎球菌分离株上的PspA。一些PspA分子与两种抗体反应，但大多数仅与一种或另一种反应。在本研究中，我们证明了PspA由所有肺炎球菌产生，证实了我们的假设，即存在Xi64和Xi26未检测到的PspA变体。我们为这些研究制备了兔抗血清和五种另外的PspA特异性单克隆抗体。兔抗血清与测试的95种肺炎球菌分离株中的每一种都发生反应，其中包括16种被膜血清型。七种单克隆抗PspA抗体中的一种或多种与95%（57种中的53种）的测试肺炎球菌分离株反应。以两种方式证实了单克隆和多克隆抗体对PspA的特异性：（i）通过检测野生型肺炎球菌上的分子，所述分子在分子量上与用Xi64和Xi26在蛋白质印迹（免疫印迹）中检测到的分子相同，和（ii）通过使用不能产生PspA或产生截短形式的PspA的肺炎链球菌突变体。用这7株单克隆抗体对57株PspA进行了鉴定，共鉴定出31种PspA亚型。对53株与单克隆抗体反应的肺炎球菌进行荚膜型、血清型和PspA分子量分析，共观察到50种不同的肺炎球菌克隆型。
Pneumococcal surface protein A (PspA) has been shown previously to elicit antibodies protective against pneumococcal infection and to be necessary for full pneumococcal virulence in mice. The protein was originally defined by the two mouse monoclonal antibodies Xi64 and Xi126, which together recognized PspA on 14% of pneumococcal isolates. Some PspA molecules reacted with both antibodies, but most reacted with only one or the other. In the present study we demonstrated that PspA is produced by all pneumococci, confirming our hypothesis that there are variants of PspA which are not detected by Xi64 and Xi26. We produced a rabbit antiserum and five additional monoclonal antibodies specific for PspA for these studies. The rabbit antiserum reacted with each of 95 pneumococcal isolates tested, comprising 16 capsular serotypes. One or more of the seven monoclonal anti-PspA antibodies reacted with 95% (53 of 57) of pneumococcal isolates tested. The specificity of the monoclonal and polyclonal antibodies to PspA was confirmed in two ways: (i) by detection of molecules on wild-type pneumococci that are identical in molecular weight to those detected in Western blots (immunoblots) with Xi64 and Xi26 and (ii) by the use of mutants of Streptococcus pneumoniae that fail to produce PspA or that produce a truncated form of PspA. By using the seven monoclonal antibodies, we observed 31 PspA types among the 57 isolates. When the 53 strains reactive with the monoclonal antibodies were analyzed by capsular type as well as by serologic type and molecular weight of PspA, we observed 50 different clonotypes of pneumococci.