Farnesol inhibits translation to limit growth and filamentation in C. albicans and S. cerevisiae.
Farnesol inhibits translation to limit growth and filamentation in C. albicans and S. cerevisiae.
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DOI：
10.15698/mic2017.09.589
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发表时间：
2017-09-04
期刊：
影响因子：
--
通讯作者：
Ashe MP
中科院分区：
文献类型：
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作者：
Egbe NE;Dornelles TO;Paget CM;Castelli LM;Ashe MP
白色念珠菌是一种多态性酵母，其中在酵母和丝状生长之间切换的能力对于致病性至关重要。法尼醇是一种群体感应倍半萜醇，通过调节特定的信号传导和转录组分，抑制白色念珠菌中的丝状生长。在这里，我们表明，法尼醇也抑制翻译的起始步骤，在白色念珠菌和S。啤酒。与靶向真核起始因子2B（eIF2B）的杂醇醇相反，法尼醇影响mRNA与小核糖体亚基的相互作用，导致S.啤酒。因此，法尼醇靶向翻译途径中不同于杂醇醇的步骤，通过否定丝状生长来引起完全相反的生理结果。
Candida albicans is a polymorphic yeast where the capacity to switch between yeast and filamentous growth is critical for pathogenicity. Farnesol is a quorum-sensing sesquiterpene alcohol that, via regulation of specific signalling and transcription components, inhibits filamentous growth in Candida albicans. Here we show that farnesol also inhibits translation at the initiation step in both Candida albicans and S. cerevisiae. In contrast to fusel alcohols, that target the eukaryotic initiation factor 2B (eIF2B), farnesol affects the interaction of the mRNA with the small ribosomal subunit leading to reduced levels of the 48S preinitiation ribosomal complex in S. cerevisiae. Therefore, farnesol targets a different step in the translation pathway than fusel alcohols to elicit a completely opposite physiological outcome by negating filamentous growth.