Wash-free immunomagnetic detection for serum through magnetic susceptibility reduction
Wash-free immunomagnetic detection for serum through magnetic susceptibility reduction
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DOI：
10.1063/1.2536127
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发表时间：
2007-02-12
影响因子：
4
通讯作者：
Yang, H. C.
中科院分区：
文献类型：
--
作者：
Hong, Chin-Yih;Chen, W. S.;Yang, H. C.
本研究讨论了一种免洗免疫磁检测平台。该试剂是一种磁性流体，由分散在水中的生物功能化磁性颗粒组成。该检测仪器为混频磁导计。磁性纳米颗粒与特定生物靶标结合形成磁性团簇，从而降低了磁性试剂的混合频率交流磁化率。对水中蛋白质（如水c反应蛋白）的免疫测定进行了表征，并与血清中的免疫测定进行了比较。这些观察结果表明，chi（ac）还原免疫磁检测具有高方便性、高灵敏度和高特异性的优点。(c) 2007年美国物理学会。
In this study, a platform for wash-free immunomagnetic detections is discussed. The reagent is a magnetic fluid composed of biofunctionalized magnetic particles dispersed in water. The assay instrument is a mixed-frequency magnetosusceptometer. The mixed-frequency ac magnetic susceptibility chi(ac) of the magnetic reagent is reduced, as a result of the formation of magnetic clusters via the association among magnetic nanoparticles and a specific biotarget. The immunoassay detection of proteins in water, such as aqueous c-reactive protein, is characterized and is compared to that in serum. These observations demonstrate the advantages of high convenience, high sensitivity, and high specificity for chi(ac)-reduction immunomagnetic detection. (c) 2007 American Institute of Physics.