The COMPASS-Like Complex Promotes Flowering and Panicle Branching in Rice
The COMPASS-Like Complex Promotes Flowering and Panicle Branching in Rice
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类似指南针的复合物促进水稻开花和穗分枝
DOI：
10.1104/pp.17.01749
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发表时间：
2018-04-01
期刊：
影响因子：
7.4
通讯作者：
Ding, Yong
中科院分区：
文献类型：
--
作者：
Jiang, Pengfei;Wang, Shiliang;Ding, Yong
开花时间（抽穗期）和穗分枝数是决定水稻产量的重要农艺性状。开花的激活需要组蛋白甲基化，但组蛋白 3 (H3K4me3) 的 Lys 4 三甲基化在调节抽穗日期和穗发育中的作用尚不清楚。在这里，我们证明了类似 COMPASS 的复合体促进开花和圆锥花序分枝。水稻 (Oryza sativa) WD40 蛋白 OsWDR5a 与 TRITHORAX 样蛋白 OsTrx1/SET 结构域组蛋白 723 (SDG723) 相互作用，形成 COMPASS 样复合物的核心成分。通过RNA干扰降低OsWDR5a或OsTrx1表达的植物产生较少的次生分枝和较少的籽粒，并且在长日照和短日照条件下表现出抽穗日期延迟，而OsWDR5a功能的丧失导致胚胎致死。 OsWDR5a 与早期抽穗日期 1 结合以调节其 H3K4me3 和表达水平。总之，我们的结果表明，COMPASS 样复合物促进开花和穗发育，并表明 COMPASS 样复合物对 H3K4me3 水平的调节对于水稻发育至关重要。
Flowering time (heading date) and panicle branch number are important agronomic traits that determine yield in rice (Oryza sativa). The activation of flowering requires histone methylation, but the roles of trimethylation of Lys 4 of histone 3 (H3K4me3) in modulating heading date and panicle development are unclear. Here, we showed that the COMPASS-like complex promotes flowering and panicle branching. The rice (Oryza sativa) WD40 protein OsWDR5a interacts with the TRITHORAX-like protein OsTrx1/SET domain group protein 723 (SDG723) to form the core components of the COMPASS-like complex. Plants in which OsWDR5a or OsTrx1 expression was decreased by RNA interference produced fewer secondary branches and less grain and exhibited a delayed heading date under long-day and short-day conditions, whereas loss of OsWDR5a function resulted in embryo lethality. OsWDR5a binds to Early heading date 1 to regulate its H3K4me3 and expression levels. Together, our results show that the COMPASS-like complex promotes flowering and panicle development and suggest that modulation of H3K4me3 levels by the COMPASS-like complex is critical for rice development.