Microbiologic and Clinical Findings of Implants in Healthy Condition and with Peri-Implantitis
Microbiologic and Clinical Findings of Implants in Healthy Condition and with Peri-Implantitis
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DOI：
10.11607/jomi.3947
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发表时间：
2015-07-01
影响因子：
2
通讯作者：
Orlato Rossetti, Paulo Henrique
中科院分区：
文献类型：
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作者：
Canullo, Luigi;Penarrocha-Oltra, David;Orlato Rossetti, Paulo Henrique
比较健康种植体和有种植体周围炎症的种植体的临床参数及种植体周沟处、种植体连接处和邻牙的牙龈沟的微生物组成。材料和方法：我们进行了一项横断面研究，包括在健康条件下连续植入种植体的患者和有种植体周围炎的患者。对患者进行筛查的临床参数包括探诊出血、口袋深度和六个部位的斑块指数。微生物学分析的样本取自三个位置：种植体周围的沟槽、种植体连接内部的沟槽和邻近牙齿的牙龈沟。采用实时定量聚合酶链式反应(PCR)对10种微生物进行检测，包括伴生放线杆菌、牙龈卟啉单胞菌、连翘坦纳氏菌、齿状坦纳氏菌、中间普雷沃特拉菌、消化链球菌、核梭杆菌、直肠弯曲杆菌、腐蚀性艾肯氏菌和白色念珠菌。反应变量为阳性部位百分比和细菌总数。结果：健康组57例，共122颗种植体；种植体周围炎组53例，共113颗种植体。两组间在磨牙症、探诊袋深度、探诊出血和放射骨水平方面的差异有统计学意义。橙色复合体(P中间体、P微型体、F核型)在两组的三种类型中都是最常见的，种植体周炎组患病率较高。两组间患病率的差异在连接处比种植体周沟处更明显。种植周炎组在三个部位的大多数微生物的绝对载量和细菌总数都较高。同样，连接内的差异比种植体周沟处的差异大。在人群和地点之间的流行率和绝对微生物负荷以及x组地点之间的交互作用中发现了显著的交互作用。结论：种植体周围炎患者与健康人的临床和微生物学特征有一定差异。两组间的微生物学差异在连接处比种植体周沟处更明显。种植体连接作为种植体周围疾病的微生物储存库及其治疗结果的潜在作用应通过进一步的研究来证实。
To compare implants in healthy conditions and implants with peri-implantitis with regard to their clinical parameters and the microbiologic composition at the peri-implant sulcus, inside the implant connection, and the gingival sulcus of neighboring teeth. Materials and Methods: A cross-sectional study was performed including consecutive patients with implants in healthy conditions and with peri-implantitis. Clinical parameters for which patients were screened included bleeding on probing, pocket depth, and plaque index at six sites. Samples for microbiologic analysis were obtained from three locations: the peri-implant sulcus, inside the implant connection, and the gingival sulcus of neighboring teeth. Quantitative real-time polymerase chain reaction (PCR) was carried out for total counts of 10 microorganisms: Aggregatibacter actinomycetemcomitans, Porphyromona gingivalis, Tanerella forsythia, Tanerella denticola, Prevotela intermedia, Peptostreptococcus micros, Fusobacterium nucleatum, Campylobacter rectus, Eikenella corrodens, and Candida albicans. The response variables were the percentage of positive sites and total bacterial counts. Results: One hundred twenty-two implants in 57 patients were analyzed in the healthy group and 113 implants in 53 patients in the peri-implantitis group. Differences between the groups were statistically significant for bruxism, probing pocket depth, bleeding on probing, and radiographic bone level. Orange complex species (P intermedia, P micros, F nucleatum) were the most prevalent in the three types of sites for both groups, and prevalence values were higher in the peri-implantitis group. Differences in prevalence between groups were more marked inside the connection than in the peri-implant sulcus. Absolute loads of most microbes and total bacterial counts were higher for the peri-implantitis group in the three locations. Again, differences were bigger inside the connection than at the peri-implant sulcus. Significant interactions were found for prevalence and absolute microbial loads between groups and locations, and for the interaction of group x location. Conclusion: Clinical and microbiologic differences were observed between healthy subjects and those with peri-implantitis. Microbiologic differences between groups were more marked inside the connection than in the peri-implant sulcus. The potential role of the implant connection as a microbial reservoir for peri-implant diseases and in the outcome of their treatment should be confirmed with further studies.