LncRNA NONHSAT009968 inhibits the osteogenic differentiation of hBMMSCs in SA-induced inflammation via Wnt3a.
LncRNA NONHSAT009968 inhibits the osteogenic differentiation of hBMMSCs in SA-induced inflammation via Wnt3a.
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DOI：
10.1016/j.bbrc.2021.08.086
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发表时间：
2021-09
影响因子：
3.1
通讯作者：
Hong-jie Wen;Zhong Chen;Yi Cui;Yongqing Xu
中科院分区：
文献类型：
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作者：
Hong-jie Wen;Zhong Chen;Yi Cui;Yongqing Xu
骨髓炎发病机制复杂，治疗效果不理想，是骨科领域最具挑战性的疾病之一。其发生和发展的机制尚不清楚。在我们之前的研究中，我们发现长非编码RNA(LncRNA)NONHSAT009968抑制了葡萄球菌蛋白A(SPA)诱导的人骨髓间充质干细胞(HBMMSCs)的成骨分化能力，但其机制尚不清楚。本研究旨在阐明NONHSAT009968调控感染条件下人骨髓间充质干细胞成骨分化和骨缺损修复的可能机制。结果表明，Wnt3a在西班牙体外诱导的hBMMSCs成骨分化中起关键作用。此外，NONHSAT009968通过Wnt3a抑制SPA处理的hBMMSCs的成骨分化，提示lncNONHSAT009968可能通过Wnt3a抑制SA诱导的炎症中hBMMSCs的成骨分化，可能影响骨髓炎的发生发展。这项研究可能为骨髓炎和感染性骨缺损提供新的见解。
Osteomyelitis is one of the most challenging diseases in the field of orthopedics for its complex pathogenesis and unsatisfactory treatment. The mechanism underlying its occurrence and development is still unclear. In our previous study, we found that long non-coding RNA (lncRNA) NONHSAT009968 inhibited the ability of osteogenic differentiation in staphylococcal protein A (SPA)-treated human bone marrow mesenchymal stem cells (hBMMSCs), but the underlying mechanism remains unclear. The current study was aimed at elucidating the possible mechanism of NONHSAT009968 in regulating osteogenic differentiation and bone defect repairability of hBMMSCs under infection. It was revealed that Wnt3a played a key role in promoting osteogenic differentiation of hBMMSCs treated with SPAin vitro.In addition, NONHSAT009968 inhibited osteogenic differentiation of hBMMSCs treated with SPA via Wnt3a, bothin vivoandin vitro.In sum, the results suggested that lncNONHSAT009968 inhibited osteogenic differentiation of hBMMSCs in SA-induced inflammation through Wnt3a, which may have affected the occurrence and development of osteomyelitis. This study might provide novel insights regarding osteomyelitis and infectious bone defects.