Berberine Ameliorates Cold and Mechanical Allodynia in a Rat Model of Diabetic Neuropathy
Berberine Ameliorates Cold and Mechanical Allodynia in a Rat Model of Diabetic Neuropathy
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DOI：
10.1089/jmf.2012.2648
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发表时间：
2013-06-01
影响因子：
2.4
通讯作者：
Kim, Hyun Jee
中科院分区：
文献类型：
--
作者：
Kim, Si Oh;Kim, Hyun Jee
本研究采用大鼠糖尿病模型，评价了黄连素对链脲佐菌素(STZ)诱导的糖尿病后寒冷性和机械性痛觉过敏的抑制作用。用链脲佐菌素(STZ)诱导大鼠糖尿病神经病变。为了测量冷痛和机械性痛觉异常，分别使用了4度C板和von Frey灯丝。阿米替林10 mg/kg、黄连素10 mg/kg和黄连素20 mg/kg单次和多次给药均能明显减轻糖尿病引起的寒冷和机械性痛觉过敏。糖尿病大鼠肝组织丙二醛、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性较正常大鼠显著升高，而阿米替林和黄连素治疗组大鼠肝组织丙二醛、超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性较STZ对照组显著降低。黄连素20 mg/kg与阿米替林10 mg/kg治疗寒性和机械性痛觉过敏的总体疗效相似，而黄连素的抗氧化作用与相同剂量的阿米替林相似。综上所述，黄连素(10 mg/kg和20 mg/kg)对糖尿病有抗过敏作用，这可能与抗氧化作用有关。可以认为，黄连素的抗炎或抗抑郁作用可能与本研究显示的抗尿囊素作用有关。
This study evaluated the antiallodynic properties of berberine on cold and mechanical allodynia after streptozotocin (STZ)-induced diabetes using a rat model. Diabetic neuropathy was induced in rats by intraperitoneal injection of STZ. To measure cold and mechanical allodynia, a 4 degrees C plate and von Frey filament were used, respectively. Cold and mechanical allodynia induced by diabetes were significantly decreased by single and repeated intraperitoneal treatment of amitriptyline at 10 mg/kg, and berberine at 10 and 20 mg/kg. The hepatic malondialdehyde, superoxide dismutase, catalase, and glutathione peroxidase activities were significantly increased in diabetic rats as compared with those in intact rats; however, in amitriptyline-and berberine-treated rats, they were significantly decreased as compared to the STZ control. The overall effects of berberine 20 mg/kg on cold and mechanical allodynia were quite similar to those of amitriptyline 10 mg/kg, and berberine exhibited similar antioxidant effects as the same dosage of amitriptyline. In conclusion, berberine (10 and 20 mg/kg) was observed to have antiallodynic effects against diabetes, which are presumed to be associated with antioxidative effects. It can be considered that the anti-inflammatory or antidepressant capacity of berberine could contribute to the antiallonynic effects shown in this study.