Developmental expression of zebrafish emx1 during early embryogenesis.
Developmental expression of zebrafish emx1 during early embryogenesis.
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DOI：
10.1016/s1567-133x(02)00062-5
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发表时间：
2002-12-01
影响因子：
1.2
通讯作者：
Dawid, Igor B.
中科院分区：
文献类型：
--
作者：
Kawahara, Atsuo;Dawid, Igor B.
Emx家族同源异型盒基因Emx 1和Emx 2在哺乳动物胚胎嘴侧脑发育中起着重要作用。在这里，我们报告一个斑马鱼emx家族基因，emx1，这是更相似的小鼠emx1基因比以前报道的斑马鱼emx1基因，我们建议重新命名该基因emx3。emx 1的表达首先在发育中的前脑中的松果体（骨骺）原基和中间中胚层内的前肾原基中的10体节阶段周围检测到。EMX1在骨骺中的表达在其他物种中尚未报道。在受精后23小时（hpf）的浮动头（flh）突变体中，骨骺中的表达被抑制，其中骨骺的发育受损。随后，emx1在端脑中表达，如哺乳动物中所报道的，并且可以在25 hpf时在嗅基板和前脑中的一小群细胞中检测到。在中胚层中，emx1的表达逐渐集中在后原肾管在体节发生，并成为主要表达在泌尿生殖开口在25 hpf。因此，emx1显示出与emx2和emx3模式不同的独特表达模式。
Emx family homeobox genes, Emx1 and Emx2, play an essential role in rostral brain development in mammalian embryos. Here we report a zebrafish emx family gene, emx1, which is more similar to the mouse Emx1 gene than the previously reported zebrafish emx1 gene; we propose to rename that gene emx3. The expression of emx1 is first detected around the 10-somite stage in the pineal gland (epiphysis) primodium in the developing anterior brain and in the pronephric primodium within the intermediate mesoderm. emx1 expression in the epiphysis has not been reported in other species. Expression in the epiphysis is suppressed at 23 h post-fertilization (hpf) in the floating head (flh) mutant, in which development of the epiphysis is impaired. Subsequently, emx1 is expressed in the telencephalon, as reported in mammals, and can be detected in the olfactory placode and in a small group of cells in the forebrain at 25 hpf. In the mesoderm, emx1 expression is gradually concentrated in the posterior pronephric duct during somitogenesis, and becomes expressed predominantly in the urogenital opening at 25 hpf. Thus, emx1 displays a unique expression pattern that is distinct from the patterns of emx2 and emx3.