Estrogen effects on cognition and hippocampal transcription in middle-aged mice
Estrogen effects on cognition and hippocampal transcription in middle-aged mice
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DOI：
10.1016/j.neurobiolaging.2007.09.004
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发表时间：
2009-06-01
影响因子：
4.2
通讯作者：
Foster, Thomas C.
中科院分区：
文献类型：
--
作者：
Aenlle, Kristina K.;Kumar, Ashok;Foster, Thomas C.
年轻和中年雌性小鼠切除卵巢，并给予雌二醇或载体循环注射。术后第5周采用Morris水迷宫评估认知功能。年龄和治疗效应在空间训练过程中出现，例如，与年轻小鼠和中年雌二醇小鼠相比，中年小鼠在获得空间搜索策略方面表现出缺陷。根据行为特征，小鼠保持注射计划直到第7周，并在最后一次注射后24小时收集海马。提取海马RNA，利用cDNA芯片技术鉴定对年龄和雌激素有反应的基因。雌二醇治疗中年小鼠改变了与转录调控、生物合成、生长、神经保护和细胞信号通路元件相关的基因表达。代表性基因的表达谱在另一组动物中使用寡核苷酸阵列和RT-PCR确认。我们的研究结果表明，雌激素治疗可以促进中年动物在衰老过程中的海马健康。(C) 2007爱思唯尔公司版权所有。
Young and middle-aged female mice were ovariectomized and given cyclic injections of either estradiol or vehicle treatments. During the fifth week after surgery the Morris water maze was used to assess cognitive function. Age and treatment effects emerged over the course of spatial training such that middle-aged vehicle treated mice exhibited deficits in acquiring a spatial search strategy compared to younger vehicle treated mice and middle-age estradiol treated mice. Following behavioral characterization, mice were maintained on their injection schedule until week seven and hippocampi were collected 24 It after the last injection. Hippocampal RNA was extracted and genes responsive to age and estrogen were identified using cDNA microarrays. Estradiol treatment in middle-aged mice altered the expression of genes related to transcriptional regulation, biosynthesis, growth, neuroprotection, and elements of cell signaling pathways. Expression profiles for representative genes were confirmed in a separate set of animals using oligonucleotide arrays and RT-PCR. Our results indicate that estrogen treatment in middle-aged animals may promote hippocampal health during the aging process. (C) 2007 Elsevier Inc. All rights reserved.