Role of AXL expression in non-small cell lung cancer
Role of AXL expression in non-small cell lung cancer
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DOI：
10.3892/ol.2016.5356
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发表时间：
2016-12-01
期刊：
影响因子：
2.9
通讯作者：
Zhang, Qigang
中科院分区：
文献类型：
--
作者：
Qu, Xiaohan;Liu, Jinlu;Zhang, Qigang
本研究旨在探讨AXL在非小细胞肺癌（NSCLC）中的表达及其临床意义。本研究选取257例非小细胞肺癌患者，采用免疫组化、western blot和定量聚合酶链反应（qPCR）方法比较配对肺癌组织和癌旁正常肺组织中酪氨酸蛋白激酶受体UFO（AXL）的表达。应用该方法检测了35例新鲜组织中AXL基因和蛋白的表达。将小干扰RNA（siRNA）转染H1299肺癌细胞株，以抑制AXL的表达，MTT法检测AXL对细胞增殖的影响，Transwell迁移实验检测AXL对H1299肺癌细胞迁移的影响。结果发现，肺癌组织中AXL染色密度明显高于癌旁正常肺组织（55.25%比26.85%; P
The present study aimed to investigate the expression profile of AXL in non-small cell lung cancer (NSCLC) and its clinical significance. The current study included 257 NSCLC patients, tyrosine-protein kinase receptor UFO (AXL) expression in paired lung cancer and adjacent normal lung tissues of NSCLC patients were compared by immunohistochemistry, western blot analysis and quantitative polymerase chain reaction (qPCR). These methods were used to detect the expression of the AXL gene and protein in fresh tissues from 35 patients. Small interfering RNA (siRNA) was transfected into the H1299 lung cancer cell line to knock down AXL expression; the effects of AXL-siRNA on cell proliferation and migration were examined by MTT and Transwell migration assay, respectively. It was found that AXL staining density in lung cancer tissues was significantly increased compared with adjacent normal lung tissues (55.25 vs. 26.85%; P