Profile of aberrant CpG island methylation along the multistep pathway of gastric carcinogenesis
Profile of aberrant CpG island methylation along the multistep pathway of gastric carcinogenesis
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DOI：
10.1097/01.lab.0000067481.08984.3f
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发表时间：
2003-05-01
影响因子：
5
通讯作者：
Jung, HY
中科院分区：
文献类型：
--
作者：
Kang, GH;Lee, S;Jung, HY
迄今为止，已经发表了几篇关于胃癌（GC）中各种基因甲基化的报道。然而，这些研究大多集中在癌组织或单一基因，并没有提供有关癌前阶段特定基因的甲基化状态或有关特定病变中其他基因的并发甲基化的信息。我们试图研究GC（n = 80）、胃腺瘤（GA）（n = 79）、肠上皮化生（IM）（n = 57）和慢性胃炎（CG）（n = 74）的大样本集合中的多个基因的甲基化。采用甲基化特异性PCR检测APC、考克斯-2、DAP-激酶、E-cadherin、GST P1、hMLH 1、MGMT、p16、p14、RASSF 1A、THBS 1和TIMP 3等12个基因的甲基化频率。发现5种不同类型的甲基化行为：（1）仅在GC中甲基化的基因（GSTP 1和RASSF 1A）; (2)低甲基化频率的基因（
To date, several reports on methylation of various genes in gastric cancer (GC) have been published. However, most of these studies focused on cancer tissues or a single gene only and gave no information about the methylation status of specific genes in the premalignant stages or about the concurrent methylation of other genes in specific lesions. We attempted to investigate methylation of multiple genes in a large sample collection of GC (n = 80), gastric adenoma (GA) (n = 79), intestinal metaplasia (IM) (n = 57), and chronic gastritis (CG) (n = 74). We determined the methylation frequency of 12 genes, including APC, COX-2, DAP-kinase, E-cadherin, GSTP1, hMLH1, MGMT, p16, p14, RASSF1A, THBS1, and TIMP3 by methylation-specific PCR. Five different classes of methylation behaviors were found: (1) genes methylated in GC only (GSTP1 and RASSF1A);. (2) genes showing low methylation frequency (