MANUFACTURE AND UTILIZATION OF ANTIMONY PH ELECTRODES
MANUFACTURE AND UTILIZATION OF ANTIMONY PH ELECTRODES
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DOI：
10.1038/ki.1978.100
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发表时间：
1978-01-01
影响因子：
19.6
通讯作者：
CARTER, NW
中科院分区：
文献类型：
--
作者：
CAFLISCH, CR;PUCACCO, LR;CARTER, NW
本文介绍了一种制作单、双管锑pH微电极的新工艺。Sb作为pH传感器的准确性的调查结果表明，pH-电压响应是：在生理范围内，主要是其中电压被开发的溶液的H+活性的结果，部分地，定性地依赖于阴离子，通过至少CO2、O2和N气体的显著量的存在而被修改，并且通过温度的波动而被显著地抵消。Sb作为pH传感器的准确度取决于校准程序的质量。如果Sb电极要准确测定生物液体的pH值，pH校准溶液必须在温度、PO 2 [O2分压]、PN 2和缓冲阴离子类型方面与未知生物液体非常相似。描述了一种校准程序，该程序可以在测量哺乳动物肾脏近端小管液的pH值时，最大限度地减少Sb pH值估计的误差。
A new technique for manufacturing single-barreled and double-barreled Sb pH microelectrodes is described. The results of investigations into the accuracy of Sb as a pH sensor disclosed that the pH-voltage response is: within the physiologic range, principally the result of the H+ activity of the solution in which the voltage is being developed, in part, qualitatively anion-dependent, modified by the presence of significant amounts of at least CO2, O2 and N gases, and markedly offset by fluctuations in temperature. The accuracy of Sb as a pH sensor is determined by the quality of the calibration procedure. If the Sb electrode is to determine accurately the pH of a biological fluid, the pH calibration solutions must closely resemble the unknown biological fluid with respect to temperature, PO2 [O2 partial pressure], PN2 and types of buffering anions. A calibration procedure is described which can minimize errors with Sb pH estimations when measuring the pH of proximal tubular fluid of the mammalian kidney.