Self-compatibility and incompatibility in tetraploid sour cherry (Prunus cerasus L.)
Self-compatibility and incompatibility in tetraploid sour cherry (Prunus cerasus L.)
复制标题
DOI：
10.1007/s00497-002-0136-6
复制
发表时间：
2002-06-01
影响因子：
--
通讯作者：
Iezzoni, AF
中科院分区：
文献类型：
--
作者：
Hauck, NR;Yamane, H;Iezzoni, AF
配子体自交不亲和(GSI)在许多茄科植物中由于多倍体而典型地“分解”，导致自交亲和(SC)四倍体个体。酸樱桃(Prunus cerasus L.)是二倍体甜樱桃(P avium L.)杂交产生的四倍体物种。四倍体磨皮樱桃(P.ruticosa Pall.)是一个例外，由自交不亲和(SI)和SC两个个体组成。由于甜樱桃表现出GSI，其中13个S核糖核酸酶(S-RNase)被鉴定为花柱S基因座产物，本研究的目的是比较甜樱桃和酸樱桃的S等位基因功能、S-核糖核酸酶序列和连锁图谱定位，作为了解酸樱桃SI和SC遗传基础的初步步骤。对两个酸樱桃品种的S核糖核酸酶进行了克隆和序列分析。两个S-核糖核酸酶的序列与甜樱桃S-核糖核酸酶的序列相同，而另外三个S-核糖核酸酶的序列具有独一无二的序列。其中一个与甜樱桃和杏树的连锁群6相似，而另两个S-RNAs相互连锁，但未与其他标记连锁。甜樱桃和酸樱桃的种间杂交表明，酸樱桃中存在GSI，并且尽管进行了多倍化，但仍保持了对常见S等位基因的识别。有人推测，酸樱桃的自交亲和性是由非功能性S核糖核酸酶和花粉S核糖核酸酶的存在引起的，这些基因可能是自然突变产生的。
Gametophytic self-incompatibility (GSI) typically "breaks down" due to polyploidy in many Solanaceous species, resulting in self-compatible (SC) tetraploid individuals. However, sour cherry (Prunus cerasus L.), a tetraploid species resulting from hybridization of the diploid sweet cherry (P avium L.) and the tetraploid ground cherry (P. fruticosa Pall.), is an exception, consisting of both self-incompatible (SI) and SC individuals. Since sweet cherry exhibits GSI with 13 S-ribonucleases (S-RNases) identified as the stylar S-locus product, the objectives were to compare sweet and sour cherry S-allele function, S-RNase sequences and linkage map location as initial steps towards understanding the genetic basis of SI and SC in sour cherry. S-RNases from two sour cherry cultivars that were the parents of a linkage mapping population were cloned and sequenced. The sequences of two S-RNases were identical to those of sweet cherry S-RNases, whereas three other S-RNases had unique sequences. One of the S-RNases mapped to the Prunus linkage group 6, similar to its location in sweet cherry and almond, whereas two other S-RNases were linked to each other but were unlinked to any other markers. Interspecific crosses between sweet and sour cherry demonstrated that GSI exists in sour cherry and that the recognition of common S-alleles has been maintained in spite of polyploidization. It is hypothesized that self-compatibility in sour cherry is caused by the existence of non-functional S-RNases and pollen S-genes that may have arisen from natural mutations.