Tag-Free, Temperature Dependent Infrared Spectra of the GFP Chromophore: Revisiting the Question of Isomerism
Tag-Free, Temperature Dependent Infrared Spectra of the GFP Chromophore: Revisiting the Question of Isomerism
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GFP 发色团的无标签、温度依赖性红外光谱：重新审视异构现象问题
DOI：
10.1021/acs.jpca.0c07172
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Weber, J. Mathias
中科院分区：
文献类型：
--
作者：
Zagorec-Marks, Wyatt;Foreman, Madison M.;Weber, J. Mathias
我们报告的红外光谱的模式发色团的绿色荧光蛋白，在离子阱中制备的温度范围从30 K到室温。我们比较了该分子的Z和E异构体的红外光谱与预测的红外光谱的变化，我们确认该分子在低温下以Z异构体存在。我们重新审视了它是否能在这个实验的温度范围内热异构化的问题，我们没有发现异构化的证据。
We report infrared spectra of a model chromophore of green fluorescent protein, prepared in an ion trap at temperatures ranging from 30 K to room temperature. We compare the changes in the infrared spectrum with predicted infrared spectra for theZandEisomers of this molecule, and we confirm that the molecule exists as theZisomer at low temperatures. We revisit the question whether or not it can thermally isomerize in the temperature range of this experiment, and we find no evidence for isomerization.