Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls.
Monoclonal antibodies indicate low-abundance links between heteroxylan and other glycans of plant cell walls.
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DOI：
10.1007/s00425-015-2375-4
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发表时间：
2015-12
期刊：
影响因子：
4.3
通讯作者：
Knox JP
中科院分区：
文献类型：
--
作者：
Cornuault V;Buffetto F;Rydahl MG;Marcus SE;Torode TA;Xue J;Crépeau MJ;Faria-Blanc N;Willats WG;Dupree P;Ralet MC;Knox JP
针对杂木聚糖细胞壁多糖制剂的两个敏感的单抗的获得，使马铃薯块茎细胞壁中木聚糖和果胶之间以及燕麦粒细胞壁中木聚糖和阿拉伯半乳糖蛋白之间的潜在相互联系得到了鉴定。植物细胞壁是结构上不同的多糖的复杂复合体，在Muro相互联系和发育功能方面都知之甚少。单抗是一种多用途的工具，可以通过对寡糖结构的特异性识别，以高灵敏度检测细胞壁糖链。描述了用富含鼠李半乳糖醛酸寡糖(RG-I)的马铃薯细胞壁组分免疫后，针对杂木聚糖的两个新的单抗LM27和LM28的分离。LM27与杂木聚糖制剂紧密结合，LM28与杂木聚糖中广泛存在的含葡萄糖醛酸基的表位结合。有证据表明，在马铃薯块茎细胞壁中，一些葡醛酸木聚糖可能与果胶大分子相连。也有证据表明，在燕麦拼写的木聚糖中，LM27和LM28表位都与阿拉伯半乳糖蛋白相连，正如LM2阿拉伯半乳糖蛋白表位所追踪的那样。这项工作扩展了植物细胞壁中可能出现的糖间连接的知识，并描述了进一步分析这种连接的分子工具。这篇文章的在线版本(doi：10.1007/s00425-0152375-4)包含补充材料，授权用户可以使用。
The derivation of two sensitive monoclonal antibodies directed to heteroxylan cell wall polysaccharide preparations has allowed the identification of potential inter-linkages between xylan and pectin in potato tuber cell walls and also between xylan and arabinogalactan-proteins in oat grain cell walls. Plant cell walls are complex composites of structurally distinct glycans that are poorly understood in terms of both in muro inter-linkages and developmental functions. Monoclonal antibodies (MAbs) are versatile tools that can detect cell wall glycans with high sensitivity through the specific recognition of oligosaccharide structures. The isolation of two novel MAbs, LM27 and LM28, directed to heteroxylan, subsequent to immunisation with a potato cell wall fraction enriched in rhamnogalacturonan-I (RG-I) oligosaccharides, is described. LM27 binds strongly to heteroxylan preparations from grass cell walls and LM28 binds to a glucuronosyl-containing epitope widely present in heteroxylans. Evidence is presented suggesting that in potato tuber cell walls, some glucuronoxylan may be linked to pectic macromolecules. Evidence is also presented that suggests in oat spelt xylan both the LM27 and LM28 epitopes are linked to arabinogalactan-proteins as tracked by the LM2 arabinogalactan-protein epitope. This work extends knowledge of the potential occurrence of inter-glycan links within plant cell walls and describes molecular tools for the further analysis of such links. The online version of this article (doi:10.1007/s00425-015-2375-4) contains supplementary material, which is available to authorised users.