Antibody, but not B-cell-dependent antigen presentation, plays an essential role in preventing Chlamydia systemic dissemination in mice
Antibody, but not B-cell-dependent antigen presentation, plays an essential role in preventing Chlamydia systemic dissemination in mice
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DOI：
10.1002/eji.201948391
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发表时间：
2020-05-01
影响因子：
5.4
通讯作者：
Li, Lin-Xi
中科院分区：
文献类型：
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作者：
Malaviarachchi, Priyangi A.;Mercado, Miguel A. B.;Li, Lin-Xi
专性细胞内细菌沙眼衣原体导致全世界最普遍的细菌性传播感染。在小鼠模型中，CD 4 T细胞在针对衣原体女性生殖道（FRT）感染的保护性免疫中起核心作用，而B细胞被认为是解决原发性衣原体感染的关键。我们最近报道了一个意想不到的要求B细胞在局部衣原体特异性CD 4 T细胞引发和细菌遏制内的FRT。在这里，我们试图解决精确的效应功能的B细胞在衣原体原发感染。使用缺乏B细胞依赖性Ag呈递（MHCIIB-/-）或缺乏循环抗体（AID（-/-）x mu S-/-）的混合骨髓嵌合体，我们表明衣原体特异性CD 4 T细胞扩增不依赖于B细胞的Ag呈递。重要的是，我们证明了抗体，而不是B细胞依赖性的Ag呈递，是防止衣原体FRT感染后全身细菌传播所必需的。
The obligate intracellular bacterium Chlamydia trachomatis causes the most prevalent bacterial sexually transmitted infection worldwide. CD4 T cells play a central role in the protective immunity against Chlamydia female reproductive tract (FRT) infection, while B cells are thought to be dispensable for resolution of primary Chlamydia infection in mouse models. We recently reported an unexpected requirement of B cells in local Chlamydia-specific CD4 T-cell priming and bacterial containment within the FRT. Here, we sought to tackle the precise effector function of B cells during Chlamydia primary infection. Using mixed bone marrow chimeras that lack B-cell-dependent Ag presentation (MHCIIB-/-) or devoid of circulating antibodies (AID(-/-) x mu S-/-), we show that Chlamydia-specific CD4 T-cell expansion does not rely on Ag presentation by B cells. Importantly, we demonstrate that antibody, but not B-cell-dependent Ag presentation, is required for preventing systemic bacterial dissemination following Chlamydia FRT infection.