Dose effects of beta-tricalcium phosphate nanoparticles on biocompatibility and bone conductive ability of three-dimensional collagen scaffolds
Dose effects of beta-tricalcium phosphate nanoparticles on biocompatibility and bone conductive ability of three-dimensional collagen scaffolds
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DOI：
10.4012/dmj.2016-295
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发表时间：
2017-10-01
影响因子：
2.5
通讯作者：
Kawanami, Masamitsu
中科院分区：
文献类型：
--
作者：
Murakami, Shusuke;Miyaji, Hirofumi;Kawanami, Masamitsu
据报道，包被β -磷酸三钙纳米颗粒的三维胶原支架具有良好的生物活性和生物降解性。然后观察（β - tcp）纳米颗粒对生物相容性和骨形成能力的剂量效应。胶原蛋白支架分别加入1、5、10和25 wt%的β - tcp纳米颗粒分散体，分别命名为TCP1、TCP5、TCP10和TCP25。β - tcp纳米颗粒的抗压强度、钙离子释放量和酶抗性随β - tcp剂量的增加而增加。TCP5在大鼠皮下组织中表现出良好的细胞向内生长行为。应用TCP10时，成骨细胞增殖和大鼠颅骨增强均明显高于其他任何支架。TCP10的骨面积比未处理的支架大7.7倍。相反，TCP25一直表现出不利的生物学效应。这些结果表明，(β - tcp纳米颗粒的应用剂量会影响支架的生物性能，因此TCP10的骨传导能力显著。
Three-dimensional collagen scaffolds coated with beta-tricalcium phosphate (beta-TCP) nanoparticles reportedly exhibit good bioactivity and biodegradability. Dose effects of (beta-TCP nanoparticles on biocompatibility and bone forming ability were then examined. Collagen scaffold was applied with 1, 5, 10, and 25 wt% beta-TCP nanoparticle dispersion and designated TCP1, TCP5, TCP10, and TCP25, respectively. Compressive strength, calcium ion release and enzyme resistance of scaffolds with (beta-TCP nanoparticles applied increased with beta-TCP dose. TCP5 showed excellent cell-ingrowth behavior in rat subcutaneous tissue. When TCP10 was applied, osteoblastic cell proliferation and rat cranial bone augmentation were greater than for any other scaffold. The bone area of TCP10 was 7.7-fold greater than that of non-treated scaffold. In contrast, TCP25 consistently exhibited adverse biological effects. These results suggest that the application dose of (beta-TCP nanoparticles affects the scaffold bioproperties; consequently, the bone conductive ability of TCP10 was remarkable.