Transmural saturation transfer analysis of the creatine kinase system in the mammalian heart.
Transmural saturation transfer analysis of the creatine kinase system in the mammalian heart.
复制标题
哺乳动物心脏肌酸激酶系统的透壁饱和转移分析。
DOI：
10.1002/mrm.1910300103
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发表时间：
1993
影响因子：
3.3
通讯作者：
Hamlin,RL
中科院分区：
文献类型：
--
作者：
Robitaille,PM;Abduljalil,A;Rath,D;Zhang,H;Hamlin,RL
结合31P核磁共振空间定位和饱和转移技术，实现了前向肌酸激酶(CK)比率的跨壁测量(ATP：肌酸N-磷酸转移酶，EC 2.7.3.2)。在丹参素心肌细胞中。使用空间定位体素同时检测左心室(LV)五个心外膜向心内膜区域。尽管室内CP/ATP比值不变，但假一级速率常数(K‘)和前向肌酸激酶速率(RT)在整个室壁显示出61%的变化。由于CK水平和计算的[ADP]、[CP]和pH在正常左心室是跨壁不变的，因此观察到的Rt变化不能用这些底物和肌酸激酶绝对水平的变化来解释。此外，由于已知心肌耗氧速率在心内膜较高，这些结果暗示正向肌酸激酶速率与氧化磷酸化速率没有直接关系。
31P NMR spatial localization and saturation transfer techniques were combined to enable the transmural measurement of the forward creatine kinase (CK) rate (ATP:creatineN‐phos‐photransferase, EC 2.7.3.2.) in thein vivocanine myocardium. Five epicardial towards endocardial regions of the left ventricle (LV) were simultaneously examined using spatially localized voxels. Although intralefl ventricular CP/ATP ratios were constant, the pseudo first order rate constant (K′) and the forward creatine kinase rate (Rt) displayed a 61% variation across the LV wall. Because CK levels and calculated [ADP], [CP]and pH are transmurally invariant in the normal left ventricle, the observed changes in the Rtcould not be explained by changes in the absolute levels of these substrates and of creatine kinase. In addition, because myocardial oxygen consumption rates are known to be higher in the endocardium, these results imply that forward creatine kinase rates are not directly related to oxidative phosphorylation rates.