Clinical significance of amyloid precursor protein in patients with testicular germ cell tumor.
Clinical significance of amyloid precursor protein in patients with testicular germ cell tumor.
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DOI：
10.1155/2013/348438
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发表时间：
2013
影响因子：
1.4
通讯作者：
Inoue S
中科院分区：
文献类型：
--
作者：
Yamada Y;Fujimura T;Takahashi S;Takayama K;Urano T;Murata T;Obinata D;Ouchi Y;Homma Y;Inoue S
导论.淀粉样前体蛋白（APP）的生物学作用还不清楚，特别是在睾丸生殖细胞肿瘤（TGCT）中。因此，我们的目的是研究APP在TGCT中的免疫反应性（IR）和表达，并评估其临床意义。材料和方法。我们对1985年至2004年获得的64例睾丸标本和21例速冻标本中APP的免疫组织化学和mRNA表达进行了分析。然后，我们评估了APP表达和TGCT临床病理状态之间的关联。结果APP IR在生殖细胞肿瘤（SGCTs）和非生殖细胞肿瘤（NGCTs）中的阳性率分别为9.8%（4/41）和39.1%（9/23）。NGCTs中IR阳性例数（P = 0.00870）和mRNA表达水平均显著高于SGCTs（P = 0.0140）。APP IR阳性还与甲胎蛋白（αFP）升高（P = 0.00870）和静脉侵犯（P = 0.0414）显著相关。结论我们观察到APP在TGCT中的表达升高，尤其是在NGCT中。APP可能与TGCT中更具侵袭性的癌症相关。
Introduction. The biological role of amyloid precursor protein (APP) is not well understood, especially in testicular germ cell tumors (TGCTs). Therefore, we aimed to investigate the immunoreactivity (IR) and expression of APP in TGCTs and evaluated its clinical relevance. Materials and Methods. We performed an analysis of immunohistochemistry and mRNA expression of APP in 64 testicular specimens and 21 snap-frozen samples obtained from 1985 to 2004. We then evaluated the association between APP expression and clinicopathological status in TGCTs. Results. Positive APP IR was observed in 9.8% (4/41) of seminomatous germ cell tumors (SGCTs) and 39.1% (9/23) of nonseminomatous germ cell tumors (NGCTs). NGCTs showed significantly more cases of positive IR (P = 0.00870) and a higher mRNA expression level compared with those of SGCTs (P = 0.0140). Positive APP IR was also significantly associated with α-fetoprotein (αFP) elevation (P = 0.00870) and venous invasion (P = 0.0414). Conclusion. We observed an elevated APP expression in TGCTs, especially in NGCTs. APP may be associated with a more aggressive cancer in TGCTs.