Phenol, a potent stimulator of adenylate cyclase in human thyroid membranes.
Phenol, a potent stimulator of adenylate cyclase in human thyroid membranes.
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苯酚，人甲状腺膜中腺苷酸环化酶的有效刺激剂。
DOI：
10.1080/07435808109049840
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Ingbar,SH
中科院分区：
文献类型：
--
作者：
Amir,SM;Mulrow,NJ;Ingbar,SH
在测试了几种商业 hCG 制剂刺激人甲状腺颗粒部分中腺苷酸环化酶的能力时，只有一种药物制剂 (APL) 具有活性。该制剂的活性在透析过程中丧失，但可以通过添加苯酚和部分添加苯甲醇（APL 中存在的两种添加剂）来完全恢复。苯酚本身（0.040-2.0 mg/ml）可对人甲状腺颗粒部分中的腺苷酸环化酶活性产生有效的、剂量依赖性的刺激，并且在大鼠肝和肾皮质的质膜中也具有活性。苯酚对 [125I]-bTSH 与人甲状腺膜的结合发挥双相作用。 0.33 至 3.3 mg/ml 之间的浓度对结合有刺激作用，而浓度较高则有抑制作用。
Among several commercial hCG preparations tested for their ability to stimulate adenylate cyclase in a human thyroid particulate fraction, only a pharmaceutical preparation (APL) was active. Activity of this preparation was lost during dialysis, but could be restored fully by the addition of phenol and partially by the addition of benzyl alcohol, the two additives present in APL. Phenol itself (0.040–2.0 mg/ml) induced a potent, dose-dependent stimulation of adenylate cyclase activity in a human thyroid particulate fraction and was also active in plasma membranes from rat liver and kidney cortex. Phenol exerted a biphasic effect on [125I]-bTSH binding to human thyroid membranes. Concentrations between 0.33 and 3.3 mg/ml were stimulatory to binding, while higher concentrations were inhibitory.