Factors affecting the size distribution of liposomes produced by freeze-thaw extrusion
Factors affecting the size distribution of liposomes produced by freeze-thaw extrusion
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DOI：
10.1016/s0378-5173(99)00207-0
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发表时间：
1999-10-15
影响因子：
5.8
通讯作者：
Taylor, KMG
中科院分区：
文献类型：
--
作者：
Castile, JD;Taylor, KMG
本文描述了一种使用冻融挤出方法生产脂质体的方案的开发。激光衍射粒度分析表明，冻融卵磷脂酰胆碱多层囊泡（eggPC MLV）的中位直径增加时，胆固醇包括在双层。使用在液氮中在-196 ° C冷冻3分钟，然后在50 ° C解冻3分钟的冷冻-解冻循环，导致卵PC/胆固醇制剂的颗粒尺寸非常大。当脂质体反复冻融时，在5次冻融循环后达到最大尺寸。脂质体在氯化钠溶液中的分散促进冻融后的大小增加，表明囊泡已聚集或融合。泊洛沙姆P338和P407抑制了在冻融过程中观察到的分散在1.0 M NaCl中的卵PC MLV的大小增加，可能是通过空间阻止聚集和融合。(C)1999 Elsevier Science B. V.保留所有权利。
This paper describes the development of a protocol for the production of liposomes using a freeze-thaw extrusion methodology. Laser diffraction particle size analysis showed that the median diameter of freeze-thawed egg phosphalidylcholine multilamellar vesicles (eggPC MLVs) was increased when cholesterol was included in the bilayers. Using a freeze-thaw cycle of 3 min freezing in liquid nitrogen at - 196 degrees C followed by 3 min thawing at 50 degrees C resulted in an anomalously large particle size for eggPC/cholesterol formulations. When liposomes were repeatedly freeze-thawed a maximum size was achieved after five freeze-thaw cycles. Dispersion of liposomes in sodium chloride solutions promoted size increases following freeze-thawing, suggesting that vesicles had aggregated or fused. Poloxamers P338 and P407 inhibited the size increases observed during freeze-thawing for eggPC MLVs dispersed in 1.0 M NaCl, probably through steric prevention of aggregation and fusion. (C) 1999 Elsevier Science B.V. All rights reserved.