A microfluidic chip based liquid-liquid extraction system with microporous membrane
A microfluidic chip based liquid-liquid extraction system with microporous membrane
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DOI：
10.1016/j.aca.2005.06.028
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发表时间：
2006-01-18
影响因子：
6.2
通讯作者：
Fang, ZL
中科院分区：
文献类型：
--
作者：
Cai, ZX;Fang, Q;Fang, ZL
开发了一种基于微加工芯片的液液萃取的稳健且简单的方法，用于芯片上样品预处理。基于芯片的提取系统由两块微加工玻璃板组成，中间夹有微孔膜。使用环氧树脂的简单粘合方法用于实现 L-L 提取芯片的粘合和缩放。利用重力驱动水流和有机流通过萃取系统中的单独通道，并通过膜分离。在萃取过程中，水性样品流中的分析物通过膜转移到有机流中。通过激光诱导荧光检测系统对提取到有机流中的分析物的荧光强度进行原位监测。使用丁基罗丹明 B (BRB) 和异丁醇的水溶液分别作为样品和萃取剂证明了该系统的性能。该系统被证明是实现基于芯片的微孔膜液液萃取的有效手段。荧光测量的精度为 1.5% R.S.D. （n = 4）。通过回归方程：l = 8.00 X 10(6) C+4.91 获得 I X 10(-7) 至 I X 10(-4) M BRB 的线性响应范围。约富集因子。 3的提取效率为69%。 (c) 2005 年 Elsevier B.V. 出版
A robust and simple approach for microfabricated chip based liquid-liquid extraction was developed for on-chip sample pretreatment. The chip based extraction system was composed of two microfabricated glass plates with a microporous membrane sandwiched in between. A simple bonding approach using epoxy was used to achieve bonding and scaling of the L-L extraction chip. Gravity was employed to drive the aqueous and organic flows through separate channels in the extraction system, separated by the membrane. During extraction, the analyte in an aqueous sample stream was transferred through the membrane into the organic stream. The fluorescence intensity of the analyte extracted into the organic stream was monitored in situ by a laser induced fluorescence detection system. The performance of the system was demonstrated using an aqueous solution of butyl rhodamine B (BRB) and isobutanol as sample and extractant, respectively. The system proved to be an efficient means for achieving chip based microporous membrane liquid-liquid extraction. The precision of fluorescence measurements was 1.5% R.S.D. (n = 4). A linear response range of I X 10(-7) to I X 10(-4) M BRB was obtained with a regression equation: l = 8.00 X 10(6) C+4.91. An enrichment factor of ca. 3 was obtained with an extraction efficiency of 69%. (c) 2005 Published by Elsevier B.V.