Identification of novel HrpXo regulons preceded by two cis-acting elements, a plant-inducible promoter box and a-10 box-like sequence, from the genome database of Xanthomonas oryzae pv. oryzae
Identification of novel HrpXo regulons preceded by two cis-acting elements, a plant-inducible promoter box and a-10 box-like sequence, from the genome database of Xanthomonas oryzae pv. oryzae
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DOI：
10.1111/j.1574-6968.2006.00265.x
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发表时间：
2006-06-01
影响因子：
2.1
通讯作者：
Tsuge, S
中科院分区：
文献类型：
--
作者：
Furutani, A;Nakayama, T;Tsuge, S
米黄单胞菌 pv 的调节蛋白 HrpXo。米曲霉是水稻细菌性叶枯病的致病因子，已知它能控制编码 III 型分泌系统成分和一些效应蛋白基因的 hrp 基因的表达。在这项研究中，我们从 X. oryzae pv 的基因组数据库中筛选了新的 HrpXo 调节子。 oryzae，搜索前面有两个预测序列基序的 ORF，即植物诱导启动子盒状序列和 -10 盒状序列。使用 gus 报告系统，15 个 ORF 候选物中的 9 个以 HrpXo 依赖性表达。我们还通过碱基取代诱变表明，这两个基序对于基因的表达都是必需的。
A regulatory protein HrpXo of Xanthomonas oryzae pv. oryzae, the causal agent of bacterial leaf blight of rice, is known to control the expression of hrp genes that encode components of a type III secretion system and of some effector protein genes. In this study, we screened novel HrpXo regulons from the genome database of X. oryzae pv. oryzae, searching for ORFs preceded by two predicted sequence motifs, a plant-inducible promoter box-like sequence and a -10 box-like sequence. Using a gus reporter system, nine of 15 ORF candidates were expressed HrpXo dependently. We also showed by base-substituted mutagenesis that both motifs are essential for the expression of the genes.