Immunoregulomics : a serum autoantibody-based platform for transcription factor profiling.
Immunoregulomics : a serum autoantibody-based platform for transcription factor profiling.
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免疫调节组学：基于血清自身抗体的转录因子分析平台。
DOI：
10.1007/978-1-60327-047-2_11
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Liu,BrianC-S
中科院分区：
文献类型：
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作者：
Tassinari,OliverW;Aponte,Margarita;CaiazzoJr,RobertJ;Liu,BrianC-S
基因表达是由一组被称为转录因子（tf）的蛋白质调控的。通过结合特定的dna -元件，每个TF在基因表达中发挥不同的功能作用。Panomics™开发了transsignal™TF - TF相互作用阵列，使用户能够与多个其他TF确定感兴趣的TF复合物。该过程的工作原理是使用特异性抗体将顺式元件结合到天然细胞核提取物tf上，并将相应的顺式元件杂交到具有不同共识序列的膜阵列上。在本方案中，我们采用该技术来表征和比较有疾病和无疾病患者对tf的自身抗体反应性。使用Panomics™组合DNA/蛋白质阵列，在膜上发现超过300个不同的元素，我们可以监测自身免疫靶向调节性tf的差异，我们将这一过程称为免疫调节组学。这种方法允许对一些可量化数据的相互作用进行定性分析。这些发现可以通过凝胶移位实验进行验证。自身抗体;interactome微阵列;转录因子。
Gene expression is regulated by a group of proteins known as transcription factors (TFs). By binding to specific DNAcis-elements, each TF contributes a different functional role in gene expression. Panomics™ has developed a TranSignal™ TF–TF Interaction Array, which enables the user to determine TF complexes of interest with multiple other TFs. The process works by immunoprecipitatingcis-elements bound to native cell nuclear extract TFs using specific antibodies to the TFs, and hybridizing the correspondingcis-elements to a membrane array spotted with different consensus sequences. In this protocol, we adapt this technology to characterize and compare autoantibody reactivity to TFs between patients with and without disease. Using Panomics™ combination DNA/protein arrays with over 300 differentcis-elements spotted on the membrane, we can monitor the differences in autoimmune-targeted regulatory TFs, a process we termed immunoregulomics. This method allows for a qualitative analysis of the interactions with some quantifiable data. The findings can then be verified with the use of gel-shift experiments. Autoantibodies; interactome microarrays; transcription factors.