Endogenous insulin-like growth factors regulate the proliferation of TSH-independent mutants derived from FRTL5 cells
Endogenous insulin-like growth factors regulate the proliferation of TSH-independent mutants derived from FRTL5 cells
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DOI：
10.1016/s0300-9084(99)80083-2
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发表时间：
1999-04-01
期刊：
影响因子：
3.9
通讯作者：
Tramontano, D
中科院分区：
文献类型：
--
作者：
Chieffi, P;Picascia, A;Tramontano, D
来源于FRTL 5细胞的TSH非依赖性突变体克隆（M细胞）在TSH不存在的情况下剧烈增殖。M细胞的生长被IGF-I以剂量依赖的方式刺激，但它不受TSH的影响。Sm1.2是一种与IGF-II交叉反应的IGF-I抗体，可显著降低M细胞的基础DNA合成。I-125-IGF-I与M细胞的结合显著低于与FRTL 5细胞的结合。M细胞在其培养基中产生IGF样肽，其似乎影响其基础DNA合成和I型受体结合外源性IGF-I的可用性。(C)法国生物化学与分子生物学协会/爱思唯尔，巴黎。
TSH-independent mutant clones (M cells) derived from FRTL5 cells, proliferate vigorously in the absence of TSH. The growth of M cells is stimulated by IGF-I in a dose-dependent fashion, but it is not influenced by TSH. Sm1.2, an antibody against IGF-I cross-reacting with IGF-II, significantly decreases basal DNA synthesis in the M cells. Binding of I-125-IGF-I to M cells is significantly lower than that to FRTL5 cells. M cells produce in their culture medium IGF-like peptides which appear to influence their basal DNA synthesis and the availability of type I receptors to bind exogenous IGF-I. (C) Societe francaise de biochimie et biologie moleculaire / Elsevier, Paris.