Potentiality of Herbaspirillum seropedicae as a Platform for Bioplastic Production
Potentiality of Herbaspirillum seropedicae as a Platform for Bioplastic Production
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DOI：
10.1007/978-981-10-2555-6_2
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发表时间：
2016-01-01
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影响因子：
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通讯作者：
de Souza, Emanuel Maltempi
中科院分区：
文献类型：
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作者：
Batista, Marcelo Bueno;Mueller-Santos, Marcelo;de Souza, Emanuel Maltempi
聚羟基丁酸酯(PHB)是一种由几种细菌产生的聚羟基烷酸酯，用作碳汇和还原当量汇。由于PHB的性质类似于石油基塑料，而PHB在环境中很容易降解，因此PHB的生产是生物技术研究的热点。因此，PHB是合成塑料材料的一种生物可持续替代材料。PHB使用的主要障碍是大规模发酵过程的成本，与以石油为基础的工业过程相比，这一成本仍然很高。使用廉价的生物质原料和工业副产品可能会降低微生物生产PHB的成本。此外，应用代谢工程来微调代谢途径，甚至创造更有效的途径，可能会在PHB生产竞争力方面产生重要的收益。在本章中，我们将探讨血清草杆菌作为PHB生产平台的潜力，分析PHB和碳代谢相关基因的功能，为新的细菌菌株的代谢工程提出策略。
Polyhydroxybutyrate (PHB) is a polyhydroxyalkanoate produced by several bacteria as carbon storage and reducing equivalent sink. The production of PHB is a hot topic in biotechnological research due to its properties similar to oil-based plastics, while PHB is readily degradable in the environment. Therefore, PHB is a bio-sustainable alternative for synthetic plastic materials. The main hurdle for PHB use is the cost of fermentative process in large scale, which is still high when compared to industrial processes based in petroleum. The use of cheap biomass feedstocks and industrial by-products can potentially reduce costs of microbial PHB production. In addition, the application of metabolic engineering to fine-tune metabolic pathways or even create more efficient pathways may yield important gains in PHB production competiveness. In this chapter we will address the potential of the bacterium Herbaspirillum seropedicae as a platform for PHB production, analyzing the functions of genes involved in PHB and carbon metabolism to propose strategies for metabolic engineering of new bacterial strains.