Effect of the combination of a cyclooxygenase-1 selective inhibitor and taxol on proliferation, apoptosis and angiogenesis of ovarian cancer in vivo.
Effect of the combination of a cyclooxygenase-1 selective inhibitor and taxol on proliferation, apoptosis and angiogenesis of ovarian cancer in vivo.
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环氧合酶-1选择性抑制剂与紫杉醇联用对体内卵巢癌增殖、凋亡和血管生成的影响。
作者：
Wei Li;Meihan Liu;Jia;Yun;L. Zhai;Jun Zhang
本研究旨在研究环氧合酶（考克斯）-1抑制剂SC-560与紫杉醇联合给药对SKOV-3人卵巢癌细胞异种移植小鼠模型抗肿瘤疗效的分子机制的影响。小鼠接受6 mg/kg/天SC-560灌胃给药，每隔一天两次，并每周一次腹腔注射20 mg/kg紫杉醇，持续3周。应用免疫组化和逆转录-聚合酶链反应（RT-PCR）分别检测卵巢癌组织中微血管密度（MVD）和血管内皮生长因子（VEGF）mRNA水平。分别采用Ki-67染色和末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）法检测肿瘤组织中增殖和凋亡细胞的指数。给药结束后第7天，联合用药组小鼠肿瘤体积较对照组缩小55.35%，差异有统计学意义（P<0.05）。联合用药组VEGF表达、MVD及细胞增殖指数均明显抑制，而凋亡指数明显升高（与对照组比较均P<0.01）。我们的研究结果表明，SC-560与紫杉醇联合治疗的抗肿瘤作用的分子机制可能部分通过抑制肿瘤血管生成，减少细胞增殖和诱导细胞凋亡而起作用。
This study was designed to investigate the effects of a cyclooxygenase (COX)-1 inhibitor, SC-560, administered in combination with taxol, on the molecular mechanisms of antitumor efficacy in a SKOV-3 human ovarian carcinoma cell xenograft-bearing mouse model. The mice were treated with 6 mg/kg/day SC-560 by gavage twice every other day and 20 mg/kg taxol by intraperitoneal injection once a week for three weeks. Microvessel density (MVD) and vascular endothelial growth factor (VEGF) mRNA levels of ovarian cancer were detected in the tumor tissues using immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR), respectively. The index of proliferating and apoptotic cells in the tumor tissues was determined by staining for Ki-67 and using the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method, respectively. On day 7 after the end of administration, the tumor volume of mice in the combination group was reduced by 55.35% compared with that of the control mice, and the difference was statistically significant (P<0.05). In the combination group, the expression of VEGF, MVD and the cell proliferation index were inhibited significantly, while the apoptotic index was notably increased (all P<0.01, compared with the control group). Our results indicate that the molecular mechanisms of the antitumor efficacy of SC-560 combined with taxol therapy may act in part by inhibiting tumor angiogenesis, reducing cell proliferation and inducing cell apoptosis.
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雌性小鼠生殖中环氧合酶 1 和环氧合酶 2 功能的药理学和/或遗传破坏的比较分析。
影响因子：
4.8
作者：
Reese,J;Zhao,X;Ma,WG;Brown,N;Maziasz,TJ;Dey,SK
通讯作者：
Dey,SK
塞来昔布可在体内防止肿瘤生长，而不对正常肠道产生毒性：体外和体内模型之间缺乏相关性。
DOI：
--
发表时间：
2000
期刊：
Cancer research.
影响因子：
--
作者：
Williams,CS;Watson,AJ;Sheng,H;Helou,R;Shao,J;DuBois,RN
通讯作者：
DuBois,RN
影响因子：
11.2
作者：
Rajnish A. Gupta;Lovella Tejada;Beverly J. Tong;S. Das;J. Morrow;S. Dey;R. Dubois
通讯作者：
Rajnish A. Gupta;Lovella Tejada;Beverly J. Tong;S. Das;J. Morrow;S. Dey;R. Dubois
影响因子：
11.2
作者：
Daikoku, T;Tranguch, S;Dey, SK
通讯作者：
Dey, SK
影响因子：
11.2
作者：
Daikoku, T;Wang, DZ;Dey, SK
通讯作者：
Dey, SK