Detection of platinum species in plant material
Detection of platinum species in plant material
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植物材料中铂形态的检测
DOI：
10.1002/elps.11501601131
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
G. Tölg
中科院分区：
文献类型：
--
作者：
J. Messerschmidt;F. Alt;G. Tölg
描述了检测天然和经铂处理的草栽培提取物中铂物质的模型实验。程序步骤是培养草样品，通过超滤和冷冻干燥提取和浓缩铂物质，通过凝胶色谱法制备分离铂物质，然后进行等速电泳，以及通过吸附伏安法对分离的铂物质进行连续分析检测。等速电泳后，可以检测到铂物质的尖峰。在原生草提取物中仅发现一种铂物质（160-200 kDa）。在经铂处理的草提取物中，观察到几种铂物质的分子量范围为 1 至 > 1000 kDa。通过极其灵敏的铂测定方法（吸附伏安法；检测限，2 pg Pt 绝对值），甚至可以在等速电泳后获得的含铂级分的水平凝胶电泳的染色蛋白条带中检测到铂。
Model experiments for the detection of platinum species in extracts from native and platinum‐treated grass cultivations are described. The procedural steps are cultivation of the grass samples, extraction and concentration of the platinum species by ultrafiltration and freeze‐drying, preparative separation of the species by gel chromatography followed by isotachophoresis, and sequential analytical detection of the separated platinum species by adsorptive voltammetry. After isotachophoresis, sharp peaks of platinum species could be detected. In the native grass extract only one platinum species (160–200 kDa) was found. In the platinum‐treated grass extracts several platinum species were observed in the molecular mass range from 1 to > 1000 kDa. By an extremely sensitive platinum determination method (adsorptive voltammetry; detection limit, 2 pg Pt abs.) it was possible to detect platinum even in stained protein bands from horizontal gel electrophoresis of platinum containing fractions obtained after isotachophoresis.