An optical technique for remote focusing in microscopy
An optical technique for remote focusing in microscopy
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DOI：
10.1016/j.optcom.2007.10.007
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发表时间：
2008-02-15
影响因子：
2.4
通讯作者：
Wilson, T.
中科院分区：
文献类型：
--
作者：
Botcherby, E. J.;Juskaitis, R.;Wilson, T.
我们描述了一种新的方法的光学重新聚焦，特别是相关的共焦和多光子显微镜系统的理论。该方法避免了其他光学重聚焦系统常见的球面像差。我们表明，无像差的重新聚焦可以实现超过70 μ m的轴向扫描范围为1.4 NA的物镜透镜。由于重新聚焦是在远离样本的地方实施的，因此该方法能够实现高轴向扫描速度，而不会在物镜透镜和样本之间产生机械干扰。(C)2007 Elsevier B.V.保留所有权利。
We describe the theory of a new method of optical refocusing that is particularly relevant for confocal and multiphoton microscopy systems. This method avoids the spherical aberration that is common to other optical refocusing systems. We show that aberration-free refocusing can be achieved over an axial scan range of 70 mu m for a 1.4 NA objective lens. As refocusing is implemented remotely from the specimen, this method enables high axial scan speeds without mechanical interference between the objective lens and the specimen. (C) 2007 Elsevier B.V. All rights reserved.