The Host Stomatal Density Determines Resistance to Septoria gentianae in Japanese Gentian
The Host Stomatal Density Determines Resistance to Septoria gentianae in Japanese Gentian
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寄主气孔密度决定日本龙胆对龙胆壳针孢的抗性
DOI：
10.1094/mpmi-05-18-0114-r
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发表时间：
2019
影响因子：
3.5
通讯作者：
Fujisaki Koki
中科院分区：
文献类型：
--
作者：
Tateda Chika;Obara Kazue;Abe Yoshiko;Sekine Reiko;Nekoduka Syuuichi;Hikage Takashi;Nishihara Masahiro;Sekine Ken-Taro;Fujisaki Koki
植物气孔是寄主植物和通过气孔进入植物组织的病原体的主要战场。一组子囊菌，利用寄主植物的气孔入侵，对农业植物造成严重损害。然而，没有证据表明气孔参与了抗壳针孢侵染的防御系统。本研究从田间表现龙胆叶枯病症状的三花龙胆中分离到龙胆壳针孢20 -35（Sg 20 -35）。建立了龙胆草离体侵染体系，观察了Sg 20 - 35对龙胆草的侵染过程，并测定了其对龙胆草的毒力。与G. scabra及其种间杂种。值得注意的是，龙胆品种对Sg 20 -35的敏感性与其近轴叶表面的气孔密度有关，而与远轴叶表面的气孔密度无关。表皮图案形成因子样9（EPFL 9/STOMAGEN）肽（一种在拟南芥中控制气孔密度的小分泌肽）的处理也增加了龙胆叶近轴侧的气孔密度。因此，经处理的植物显示出对Sg 20 -35的增强的易感性。这些结果表明，近轴叶表面气孔密度是决定龙胆品种感病性的主要因素之一。因此，控制气孔密度可能是一种有效的抗裂策略。
Plant stomata represent the main battlefield for host plants and the pathogens that enter plant tissues via stomata.Septoriaspp., a group of ascomycete fungi, use host plant stomata for invasion and cause serious damage to agricultural plants. There is no evidence, however, showing the involvement of stomata in defense systems againstSeptoriainfection. In this study, we isolatedSeptoria gentianae20-35 (Sg20-35) fromGentiana triflorashowing gentian leaf blight disease symptoms in the field. Establishment of an infection system using gentian plants cultured in vitro enabled us to observe theSg20-35 infection process and estimate its virulence in several gentian cultivars or lines.Sg20-35 also entered gentian tissues via stomata and showed increased virulence inG. trifloracompared withG. scabraand their interspecific hybrid. Notably, the susceptibility of gentian cultivars toSg20-35 was associated with their stomatal density on the adaxial but not abaxial leaf surface. Treatment of EPIDERMAL PATTERNING FACTOR-LIKE 9 (EPFL9/STOMAGEN) peptides, a small secreted peptide controlling stomatal density inArabidopsis thaliana, increased stomatal density on the adaxial side of gentian leaves as well. Consequently, treated plants showed enhanced susceptibility toSg20-35. These results indicate that stomatal density on the adaxial leaf surface is one of the major factors determining the susceptibility of gentian cultivars toS. gentianaeand suggest that stomatal density control may represent an effective strategy to conferSeptoriaresistance.