Protein A-tagging for purification of native macromolecular complexes from Candida albicans

Protein A-tagging for purification of native macromolecular complexes from Candida albicans
复制标题

DOI：
10.1002/yea.1036
复制
发表时间：
2003-11-01
期刊：
YEAST
影响因子：
2.6
通讯作者：
Brown, JD
Brown, JD
中科院分区：
生物学4区
文献类型：
--
作者：
Blackwell, C;Russell, CL;Brown, JD

文献摘要

被引文献

相似文献

蛋白质A标记已成为许多系统中描述蛋白质相互作用的重要工具，使天然条件下多组分复合体的纯化成为可能。在这里，我们提供了一组载体，通过将标签添加到开放阅读框中，允许在白色念珠菌中标记蛋白A。这些载体被成功地用来产生具有功能、被适当定位或组装成复合体的稳定标记的蛋白质。这些新载体构成了白色念珠菌分子工具箱的一个有用的补充。版权所有(C)2003 John Wiley Sons，Ltd.
Protein A-tagging has become an important tool in characterization of protein-protein interactions in many systems, allowing purification of multicomponent complexes under native conditions. Here we provide a set of vectors that allow protein A-tagging in Candida albicans, through addition of the tag to open reading frames. These vectors were successfully used to generate stably tagged proteins that were functional, shown to be localized appropriately or assembled into complexes. These new vectors comprise a useful addition to the C. albicans molecular toolbox. Copyright (C) 2003 John Wiley Sons, Ltd.