iPS cells produce viable mice through tetraploid complementation
iPS cells produce viable mice through tetraploid complementation
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iPS 细胞通过四倍体互补产生可存活的小鼠
DOI：
10.1038/nature08267
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发表时间：
2009-09-03
期刊：
影响因子：
64.8
通讯作者：
Zhou, Qi
中科院分区：
文献类型：
--
作者：
Zhao, Xiao-yang;Li, Wei;Zhou, Qi
自从最初描述了通过在小鼠成纤维细胞中强制表达四种转录因子而产生的诱导多能干细胞（iPS）以来，该技术已被用于从其他物种（包括灵长类动物和大鼠）的各种细胞类型中产生胚胎干细胞（ES）样多能细胞。它已成为一种流行的手段，将体细胞基因组重编程为胚胎样多能状态，以及体细胞核移植和体细胞与ES细胞融合的优选替代方案。然而，iPS细胞重编程仍然缓慢且低效。值得注意的是，通过最严格的四倍体互补测定没有产生活动物，表明未能产生完全多能细胞。在这里，我们报告了几个iPS细胞系，能够产生可行的，肥沃的活产后代的四倍体互补的一代。这些iPS细胞保持了多能潜能，非常接近于从活体或核移植胚胎产生的ES细胞。我们证明了使用iPS细胞作为表征细胞重编程和发育潜能的有用工具的实用性，并证实iPS细胞可以达到与ES细胞相似的真正的多能性。
Since the initial description of induced pluripotent stem (iPS) cells created by forced expression of four transcription factors in mouse fibroblasts, the technique has been used to generate embryonic stem (ES)-cell-like pluripotent cells from a variety of cell types in other species, including primates and rat,,,,,. It has become a popular means to reprogram somatic genomes into an embryonic-like pluripotent state, and a preferred alternative to somatic-cell nuclear transfer and somatic-cell fusion with ES cells,. However, iPS cell reprogramming remains slow and inefficient. Notably, no live animals have been produced by the most stringent tetraploid complementation assay, indicative of a failure to create fully pluripotent cells. Here we report the generation of several iPS cell lines that are capable of generating viable, fertile live-born progeny by tetraploid complementation. These iPS cells maintain a pluripotent potential that is very close to ES cells generated fromin vivoor nuclear transfer embryos. We demonstrate the practicality of using iPS cells as useful tools for the characterization of cellular reprogramming and developmental potency, and confirm that iPS cells can attain true pluripotency that is similar to that of ES cells.