Role of adipocyte-derived apoE in modulating adipocyte size, lipid metabolism, and gene expression in vivo
Role of adipocyte-derived apoE in modulating adipocyte size, lipid metabolism, and gene expression in vivo
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DOI：
10.1152/ajpendo.90964.2008
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发表时间：
2009-05-01
影响因子：
5.1
通讯作者：
Mazzone, Theodore
中科院分区：
文献类型：
--
作者：
Huang, Zhi Hua;Gu, DeSheng;Mazzone, Theodore
黄志华，顾东，马宗涛。脂肪细胞源性apoE在体内调节脂肪细胞大小、脂质代谢和基因表达中的作用。[J] .中国生物医学工程学报，2009,31(4):559 - 559。2009年2月17日首次出版；doi: 10.1152 / ajpendo.90964.2008。从载脂蛋白E （apoE）敲除（EKO）小鼠中分离的脂肪细胞显示甘油三酯（TG）代谢和基因表达的改变。本研究旨在评估体内内源性脂肪细胞apoE对这些脂肪细胞参数的影响，而不依赖于EKO小鼠的严重紊乱的代谢环境。将野生型（WT）或EKO小鼠的脂肪组织移植到WT受体中，然后喂食饲料或高脂饮食8-10周。经鼠粮喂养后，新鲜分离的移植EKO脂肪细胞显著小于移植WT脂肪细胞（P < 0.05） （70%）， TG合成率显著降低，TG水解率显著提高。移植的EKO脂肪细胞也具有较高的脂联素、periilipin和脂肪酸氧化途径酶编码基因mRNA水平和较低的caveolin水平。在高脂肪饮食和随之而来的循环脂质和载脂蛋白e水平增加后，移植的WT脂肪细胞大小增加了106 × 103 μ m(3)，而EKO脂肪细胞大小仅增加了19 × 10 μ m(3)。从WT受体或EKO脂肪组织移植的内源性宿主脂肪组织中收获的脂肪细胞大小没有任何差异。与体内观察一致，EKO脂肪细胞与含载脂蛋白e的富含TG的脂蛋白孵育时合成的TG比WT脂肪细胞少。我们的研究结果表明，不同于循环apoE，内源性apoE在调节脂肪细胞TG代谢和基因表达方面具有新的体内作用。他们支持内源性产生的脂肪细胞apoE促进脂肪细胞从循环富含tg的脂蛋白中获取脂质的模型。
Huang ZH, Gu D, Mazzone T. Role of adipocyte-derived apoE in modulating adipocyte size, lipid metabolism, and gene expression in vivo. Am J Physiol Endocrinol Metab 296: E1110-E1119, 2009. First published February 17, 2009; doi: 10.1152/ajpendo.90964.2008. Adipocytes isolated from apolipoprotein E (apoE)-knockout (EKO) mice display alterations in triglyceride (TG) metabolism and gene expression. The present studies were undertaken to evaluate the impact of endogenously produced adipocyte apoE on these adipocyte parameters in vivo, independent of the profoundly disturbed metabolic milieu of EKO mice. Adipose tissue from wild-type (WT) or EKO mice was transplanted into WT recipients, which were then fed chow or high-fat diet for 8-10 wk. After a chow diet, freshly isolated transplanted EKO adipocytes were significantly (P < 0.05) smaller (70%) than transplanted WT adipocytes and displayed significantly lower rates of TG synthesis and higher rates of TG hydrolysis. Transplanted EKO adipocytes also had higher mRNA levels for adiponectin, perilipin, and genes coding for enzymes in the fatty acid oxidation pathway and lower levels of caveolin. After a high-fat diet and consequent increase in circulating lipid and apoE levels, transplanted WT adipocyte size increased by 106 x 103 mu m(3), whereas EKO adipocyte size increased only by 19 x 10(3) mu m(3). Endogenous host adipose tissue harvested from WT recipients of transplanted WT or EKO adipose tissue did not demonstrate any difference in adipocyte size. Consistent with the in vivo observations, EKO adipocytes synthesized less TG when incubated with apoE-containing TG-rich lipoproteins than WT adipocytes. Our results establish a novel in vivo role for endogenously produced apoE, distinct from circulating apoE, in modulation of adipocyte TG metabolism and gene expression. They support a model in which endogenously produced adipocyte apoE facilitates adipocyte lipid acquisition from circulating TG-rich lipoproteins.