Derivatization of peptides as quaternary ammonium salts for sensitive detection by ESI-MS
Derivatization of peptides as quaternary ammonium salts for sensitive detection by ESI-MS
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DOI：
10.1002/psc.1342
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发表时间：
2011-06-01
影响因子：
2.1
通讯作者：
Szewczuk, Zbigniew
中科院分区：
文献类型：
--
作者：
Cydzik, Marzena;Rudowska, Magdalena;Szewczuk, Zbigniew
采用固相合成法高效制备了一系列N端季铵盐形式的模型肽。肽季铵衍生物的串联质谱分析显示提供序列确认和增强的检测。我们设计了2-（1，4-二氮杂双环[2.2.2]辛基铵）乙酰基季铵基团，其在MS/MS实验期间不遭受中性损失。所提出的1，4-二氮杂双环[2.2.2]辛烷（DABCO）通过碘乙酰化肽的季铵化相对容易并且与标准固相肽合成相容。这种方法提供了一种新的灵敏的方法来分析肽和其他化合物。版权所有（C）2011欧洲肽协会和约翰威利父子有限公司。
A series of model peptides in the form of quaternary ammonium salts at the N-terminus was efficiently prepared by the solid-phase synthesis. Tandem mass spectrometric analysis of the peptide quaternary ammonium derivatives was shown to provide sequence confirmation and enhanced detection. We designed the 2-(1,4-diazabicyclo[2.2.2] octylammonium) acetyl quaternary ammonium group which does not suffer from neutral losses during MS/MS experiments. The presented quaternization of 1,4-diazabicyclo[2.2.2] octane (DABCO) by iodoacetylated peptides is relatively easy and compatible with standard solid-phase peptide synthesis. This methodology offers a novel sensitive approach to analyze peptides and other compounds. Copyright (C) 2011 European Peptide Society and John Wiley & Sons, Ltd.