Comparison of the functional characterization between alkane monooxygenases for low-molecular-weight polyethylene biodegradation
Comparison of the functional characterization between alkane monooxygenases for low-molecular-weight polyethylene biodegradation
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DOI：
10.1016/j.ibiod.2016.06.012
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发表时间：
2016-10-01
影响因子：
4.8
通讯作者：
Kim, Mal Nam
中科院分区：
文献类型：
--
作者：
Jeon, Hyun Jeong;Kim, Mal Nam
已知铜绿假单胞菌菌株E7的AlkB 1是用于烷烃降解的酶。据报道，AlkB 1对低分子量聚乙烯（LMWPE-1）的降解具有活性，并且在RubA 1、RubA 2和RubB的存在下降解增加。作为这些结果的延伸，本研究调查了AlkB 2对LMWPE-1降解的活性以及AlkB 2和RubAl/RubA 2之间的相互作用。将AlkB 1在LMWPE-1降解中的活性与AlkB 2的活性进行比较。对alkB 1和alkB 2在正十二烷和正十六烷诱导下的转录水平的检测表明，alkB 1的转录受正十六烷的诱导，而alkB 2的转录受正十二烷和正十六烷的诱导。正十六烷诱导的alkB 2转录水平比alkB 1高7倍左右。在LMWPE-1的存在下，alkB 2的转录也比alkB 1的转录更高并且持续时间更长。BCA降解LMWPE-1的结果（插入alkB 1），BCA 2（插入alkB 2）和BCA 2 R在堆肥条件下对重组菌株（插入alkB 2、rubA 1和rubA 2）的降解活性进行了研究，结果表明，BCA 2菌株对LMWPE-1的降解活性高于BCA菌株，和rub。BCA 2 R菌株对LMWPE-1的生物降解并不高于BCA 2菌株，尽管BCA 2 R菌株含有RubA 1和RubA 2。因此，可以得出结论，AlkB 2比AlkB 1更有效地降解LMWPE-1，并且AlkB 2的调节机制不同于AlkB 1的调节机制。(C)2016爱思唯尔有限公司版权所有。
AlkB1 of the Pseudomonas aeruginosa strain E7 is known as an enzyme that is used in alkane degradation. It has been reported that AlkB1 is active for degradation of low molecular weight polyethylene (LMWPE-1) and that degradation increased in the presence of RubA1, RubA2, and RubB. As an extension of these results, this study investigated the activity of AlkB2 toward LMWPE-1 degradation and the mutual interaction between AlkB2 and RubAl/RubA2. The activity of AlkB1 in LMWPE-1 degradation was compared with that of AlkB2. An examination of the transcription levels of alkB1 and alkB2 induced by n-dodecane and n-hexadecane indicated that the transcription of alkB1 was induced by n-hexadecane but not by n-dodecane, whereas that of alkB2 was induced by both n-dodecane and n-hexadecane. The transcription level of alkB2 induced by n-hexadecane was about 7 times higher than that of alkB1. The transcription of alkB2 was also higher and persisted longer than that of alkB1 in the presence of LMWPE-1. The results of LMWPE-1 biodegradation by BCA (inserted alkB1), BCA2 (inserted alkB2), and BCA2R (inserted alkB2, rubA1 and rubA2) recombinant strains under controlled compost conditions revealed that LMWPE-1 biodegradation by the BCA2 strain was higher than that by the BCA strain, and the biodegradation activity of the BCA2 strain was similar to that of the strain cloned with alkB1, rubA1, rubA2, and rubB. LMWPE-1 biodegradation by the BCA2R strain was not higher than that by the BCA2 strain, even though the BCA2R strain contains RubA1 and RubA2. Therefore, it was concluded that AlkB2 is more effective than AlkB1 toward LMWPE-1 degradation, and that the regulation mechanism of AlkB2 is different from that of AlkB1. (C) 2016 Elsevier Ltd. All rights reserved.