Correlations between gaseous and liquid phase chemistries induced by cold atmospheric plasmas in a physiological buffer
Correlations between gaseous and liquid phase chemistries induced by cold atmospheric plasmas in a physiological buffer
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DOI：
10.1039/c8cp00264a
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发表时间：
2018-04-14
影响因子：
3.3
通讯作者：
Clement, Franck
中科院分区：
文献类型：
--
作者：
Girard, Fanny;Peret, Mathieu;Clement, Franck
等离子体-液体相互作用的理解是非常重要的，不仅在物理化学，化学工程和聚合物科学，但在生物医学以及更好地控制生物样品上/中诱导的冷大气等离子体（CAP）的生物过程。此外，必须特别考虑等离子体-空气相互作用，因为这些CAP在环境空气中传播。在这里，我们开发了一个基于氦的CAP设置配备了屏蔽气体装置，它允许控制等离子体-空气相互作用。多亏了这个装置，我们获得了特定的漫射CAP，能够在大气压力下在环境空气中传播沿着几厘米。在这些CAP与液体介质（磷酸盐缓冲盐水PBS 10 mM，pH 7.4）相互作用期间，对它们进行光发射光谱（OES）测量，给出关于作为保护气体组成（可变O-2/（O-2 + N-2）比）的函数的诱导化学的有价值的信息。检测到几种激发态，包括N-2（+）（第一负系统，FNS），N-2（第二正系统，SPS）和HO中心点自由基。氮/氧激发物种之间的比率强烈地依赖于O-2/（O-2 + N-2）比率。采用电化学和紫外-可见吸收光谱相结合的方法研究了CAP处理后的液相化学。我们检测并定量了稳定的氧和氮物质（H2 O2、NO2-、NO3-）沿着活性氮物质（RNS）如过氧亚硝酸根阴离子ONOO中心点。似乎在处理的液体中的RNS/ROS（活性氧物质）比率也取决于保护气体组成。最终，CAP周围环境的组成似乎对诱导的等离子体化学以及因此对液体化学至关重要。所有这些结果清楚地表明，对于通常在周围空气环境中实现的物理、化学和生物医学应用，有必要实现等离子体-空气相互作用的有效控制。
The understanding of plasma-liquid interactions is of major importance, not only in physical chemistry, chemical engineering and polymer science, but in biomedicine as well as to better control the biological processes induced on/in biological samples by Cold Atmospheric Plasmas (CAPs). Moreover, plasma-air interactions have to be particularly considered since these CAPs propagate in the ambient air. Herein, we developed a helium-based CAP setup equipped with a shielding-gas device, which allows the control of plasma-air interactions. Thanks to this device, we obtained specific diffuse CAPs, with the ability to propagate along several centimetres in the ambient air at atmospheric pressure. Optical Emission Spectroscopy (OES) measurements were performed on these CAPs during their interaction with a liquid medium (phosphate-buffered saline PBS 10 mM, pH 7.4) giving valuable information about the induced chemistry as a function of the shielding gas composition (variable O-2/(O-2 + N-2) ratio). Several excited species were detected including N-2(+) (First Negative System, FNS), N-2(Second Positive System, SPS) and HO center dot radical. The ratios between nitrogen/oxygen excited species strongly depend on the O-2/(O-2 + N-2) ratio. The liquid chemistry developed after CAP treatment was investigated by combining electrochemical and UV-visible absorption spectroscopy methods. We detected and quantified stable oxygen and nitrogen species (H2O2, NO2-, NO3-) along with Reactive Nitrogen Species (RNS) such as the peroxynitrite anion ONOO center dot. It appears that the RNS/ROS (Reactive Oxygen Species) ratio in the treated liquid depends also on the shielding gas composition. Eventually, the composition of the surrounding environment of CAPs seems to be crucial for the induced plasma chemistry and consequently, for the liquid chemistry. All these results demonstrate clearly that for physical, chemical and biomedical applications, which are usually achieved in ambient air environments, it is necessary to realize an effective control of plasma-air interactions.