Rapid speciation of Se(IV) and Se(VI) by flow injection-capillary electrophoresis system with contactless conductivity detection
Rapid speciation of Se(IV) and Se(VI) by flow injection-capillary electrophoresis system with contactless conductivity detection
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DOI：
10.1007/s00216-003-2207-4
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发表时间：
2004-01-01
影响因子：
4.3
通讯作者：
Kubán, V
中科院分区：
文献类型：
--
作者：
Kubán, P;Kubán, P;Kubán, V
采用流动注射-毛细管电泳法，采用非接触式电导检测和静压流动，实现了Se(IV)和Se(VI)的快速、灵敏的形态分析。使用含有8.75mM L组氨酸(His)的背景电解质溶液，用冰醋酸调节pH至4.00，样品吞吐量为25个样品/小时。Se(IV)和Se(VI)的峰面积重复性(n=8)均优于1.41%，检出限分别为190mug L~(-1)和7.5mug L~(-1)。碳酸盐通常存在于水样中，通过使用低pH电解液消除了碳酸盐的干扰，在低pH电解液中碳酸盐不带电并在EOF前沿迁移。方法用于同时添加硒物种的土壤样品中Se(IV)和Se(VI)的分析，两种硒物种的回收率与其进样浓度一致。
A flow injection-capillary electrophoresis system with contactless conductivity detection and hydrostatic-pressure-generated flow was used for the fast and sensitive speciation of Se(IV) and Se(VI). The sample throughput was 25 samples per hour using a background electrolyte solution containing 8.75 mM L-histidine (His) adjusted to pH 4.00 with acetic acid. The repeatability of peak areas (n=8) was better than 1.41% and the limits of detection were 190 mug L-1 and 7.5 mug L-1 for Se(IV) and Se(VI), respectively. The interference from carbonate, typically present in water samples, was eliminated by using a low-pH electrolyte in which carbonate is uncharged and migrates at the EOF front. The method was applied to the analysis of Se(IV) and Se(VI) in soil samples that were spiked with both selenium species and the results for recovery of both selenium species were in good agreement with their introduced concentrations.