Characterization of Translocation of Silver Nanoparticles and Effects on Whole-Genome Gene Expression Using an In Vitro Intestinal Epithelium Coculture Model
Characterization of Translocation of Silver Nanoparticles and Effects on Whole-Genome Gene Expression Using an In Vitro Intestinal Epithelium Coculture Model
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DOI：
10.1021/nn2007145
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发表时间：
2011-05-01
期刊：
影响因子：
17.1
通讯作者：
Hendriksen, Peter J. M.
中科院分区：
文献类型：
--
作者：
Bouwmeester, Hans;Poortman, Jenneke;Hendriksen, Peter J. M.
纳米颗粒在食品领域的应用是突出的。银纳米颗粒是最常用的，使得消费者不可避免地接触到银纳米颗粒。因此，关于银纳米颗粒通过肠道吸收和可能的毒性作用的信息非常重要，但仍然缺乏。在本研究中，我们使用了一个体外模型的人肠上皮细胞组成的Caco-2和M-细胞，研究通过银纳米粒子及其离子等价物，并评估其对全基因组mRNA表达的影响。将该体外肠模型暴露于四种尺寸的银纳米颗粒4小时。包括暴露于银离子作为对照，因为发现6-17%的银纳米颗粒解离成银离子。对于银离子和纳米颗粒暴露，通过Caco-2细胞屏障的银离子的量相等。纳米颗粒在一系列应激反应中诱导基因表达的明显变化，包括氧化应激、内质网应激反应和细胞凋亡。然而，对银纳米颗粒的基因表达反应与AgNO 3非常相似。因此，观察到的银纳米颗粒的效果可能是由从纳米颗粒释放的银离子发挥的。
Applications of nanoparticles in the food sector are eminent. Silver nanoparticles are among the most frequently used, making consumer exposure to silver nanoparticles inevitable. Information about uptake through the intestines and possible toxic effects of silver nanoparticles is therefore very important but still lacking. In the present study, we used an in vitro model for the human intestinal epithelium consisting of Caco-2 and M-cells to study the passage of silver nanoparticles and their ionic equivalents and to assess their effects on whole-genome mRNA expression. This in vitro intestine model was exposed to four sizes of silver nanoparticles for 4 h. Exposure to silver ions was included as a control since 6-17% of the silver nanoparticles were found to be dissociated into silver ions. The amount of silver ions that passed the Caco-2 cell barrier was equal for the silver ion and nanoparticle exposures. The nanoparticles induced clear changes in gene expression in a range of stress responses including oxidative stress, endoplasmatic stress response, and apoptosis. The gene expression response to silver nanoparticles, however, was very similar to that of AgNO3. Therefore, the observed effects of the silver nanoparticles are likely exerted by the silver ions that are released from the nanoparticles.