NURD, a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities
NURD, a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities
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DOI：
10.1016/s1097-2765(00)80299-3
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发表时间：
1998-12-01
期刊：
影响因子：
16
通讯作者：
Wang, WD
中科院分区：
文献类型：
--
作者：
Xue, YT;Wong, JM;Wang, WD
已知ATP依赖性染色质重塑复合物通过打开染色质结构来促进转录激活。我们报告了一种新的人类复合物，命名为candidD，它不仅含有ATP依赖的核小体破坏活性，但也组蛋白脱乙酰酶活性，这通常与转录抑制。核小体模板上的ATP刺激脱乙酰化，表明核小体破坏有助于脱乙酰化酶接近其底物。一个亚基的CD 3D被确定为MTA 1，转移相关的蛋白质与核受体辅阻遏物，N-CoR相似的区域;和抗体CD 3D部分缓解甲状腺激素受体的转录抑制。这些结果表明，ATP依赖的染色质重塑可以通过协助阻遏物进入染色质来参与转录阻遏。
ATP-dependent chromatin-remodeling complexes are known to facilitate transcriptional activation by opening chromatin structures. We report a novel human complex, named NURD, which contains not only ATP-dependent nucleosome disruption activity, but also histone deacetylase activity, which usually associates with transcriptional repression. The deacetylation is stimulated by ATP on nucleosomal templates, suggesting that nucleosome disruption aids the deacetylase to access its substrates. One subunit of NURD was identified as MTA1, a metastasis-associated protein with a region similar to the nuclear receptor corepressor, N-CoR; and antibodies against NURD partially relieve transcriptional repression by thyroid hormone receptor. These results suggest that ATP-dependent chromatin remodeling can participate in transcriptional repression by assisting repressors in gaining access to chromatin.